Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection.

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Clinical proteomics Pub Date : 2023-07-01 DOI:10.1186/s12014-023-09415-y

Lydia Kollhoff, Marc Kipping, Manfred Rauh, Uta Ceglarek, Günes Barka, Frederik Barka, Andrea Sinz

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引用次数: 1

Abstract

We have developed a rapid and highly specific assay for detecting and monitoring SARS-CoV-2 infections by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). As MALDI-TOF mass spectrometers are available in a clinical setting, our assay has the potential to serve as alternative to the commonly used reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Sample preparation prior to MALDI-TOF-MS involves the tryptic digestion of SARS-CoV-2 proteins, followed by an enrichment of virus-specific peptides from SARS-CoV-2 nucleoprotein via magnetic antibody beads. Our MALDI-TOF-MS method allows the detection of SARS-CoV-2 nucleoprotein in sample collection medium as low as 8 amol/µl. MALDI-TOF mass spectra are obtained in just a few seconds, which makes our MS-based assay suitable for a high-throughput screening of SARS-CoV-2 in healthcare facilities in addition to PCR. Due to the specific detection of virus peptides, different SARS-CoV-2 variants are readily distinguished from each other. Specifically, we show that our MALDI-TOF-MS assay discriminates SARS-CoV-2 strain B.1.617.2 "delta variant" from all other variants in patients' samples, making our method highly valuable to monitor the emergence of new virus variants.

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建立一种快速、特异的MALDI-TOF质谱检测SARS-CoV-2的方法。

我们开发了一种快速和高度特异性的检测和监测SARS-CoV-2感染的基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)方法。由于MALDI-TOF质谱仪可用于临床环境，我们的检测方法有可能替代常用的逆转录酶定量聚合酶链反应(RT-qPCR)。MALDI-TOF-MS前的样品制备包括胰蛋白酶消化SARS-CoV-2蛋白，然后通过磁性抗体珠从SARS-CoV-2核蛋白中富集病毒特异性肽。我们的MALDI-TOF-MS方法允许在低至8 amol/µl的样品采集介质中检测SARS-CoV-2核蛋白。MALDI-TOF质谱仅需几秒钟即可获得，这使得我们基于质谱的检测方法除了PCR外，还适用于医疗机构中SARS-CoV-2的高通量筛选。由于病毒肽的特异性检测，不同的SARS-CoV-2变体很容易区分。具体来说，我们的MALDI-TOF-MS检测方法可以将SARS-CoV-2菌株B.1.617.2“δ变体”与患者样本中的所有其他变体区分开来，使我们的方法对监测新病毒变体的出现具有很高的价值。

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来源期刊

Clinical proteomics BIOCHEMICAL RESEARCH METHODS-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

17 weeks

期刊介绍： Clinical Proteomics encompasses all aspects of translational proteomics. Special emphasis will be placed on the application of proteomic technology to all aspects of clinical research and molecular medicine. The journal is committed to rapid scientific review and timely publication of submitted manuscripts.