Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp.: cloning, expression and characterization.

IF 1.5 4区生物学 Q4 MYCOLOGY

Mycoscience Pub Date : 2022-01-01 DOI:10.47371/mycosci.2022.09.001

Gaku Tsutsumi, Chikako Kuroki, Kengo Kamei, Mizuho Kusuda, Masami Nakazawa, Tatsuji Sakamoto, Mariko Ishikawa, Shinji Harada, Hitoshi Kobayashi, Kenji Ouchi, Satoshi Inatomi, Minoru Sakaguchi, Takeo Iwamoto, Mitsuhiro Ueda

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Abstract

The N-terminal amino acid sequence of the Pleurotus sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from P. ostreatus PC 15 v2.0. The length of the Pleurotus sp. trehalase gene was 2247 bp, encoding a protein of 749 amino acids with a predicted molecular mass of 81.2 kDa. The molecular mass of the recombinant enzyme was estimated to be about 117 kDa by SDS-PAGE. We found that the recombinant enzyme comprised an N-glycosylated sugar chain and that its optimum pH and temperature were 4.5 and 40 ºC, respectively. Moreover, this enzyme exhibited high activity against trehalose exclusively. We found that the enzyme is novel acid trehalase belonging to GH family 37.

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侧耳菌糖苷水解酶37家族新型酸性海藻糖酶的克隆、表达与特性研究。

Pleurotus sp. 90 kDa蛋白n端氨基酸序列与P. ostreatus PC 15 v2.0中糖苷水解酶(GH)家族37蛋白(海藻酶)的相应序列一致。该基因全长2247 bp，编码749个氨基酸，预测分子量为81.2 kDa。经SDS-PAGE分析，重组酶的分子量约为117 kDa。我们发现重组酶由一个n -糖基化糖链组成，其最适pH和温度分别为4.5和40℃。此外，该酶对海藻糖具有较高的特异性活性。我们发现该酶是一种新型的酸性海藻化酶，属于GH家族37。

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来源期刊

Mycoscience MYCOLOGY-

CiteScore

3.00

自引率

7.10%

发文量

审稿时长

3 months

期刊介绍： Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.