COVID-19 Analysis in Tissue Samples Acquired by Minimally Invasive Autopsy in Out-of-Hospital Deaths with Postmortem Degeneration.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2023-09-22 Epub Date: 2023-06-30 DOI:10.7883/yoken.JJID.2023.140
Yuichiro Hirata, Yohsuke Makino, Shun Iida, Harutaka Katano, Sayaka Nagasawa, Hirofumi Rokutan, Munetoshi Hinata, Akiko Iwasaki, Yoichi Yasunaga, Hiroyuki Abe, Masako Ikemura, Ayumi Motomura, Kei Kira, Susumu Kobayashi, Shigeki Tsuneya, Suguru Torimitsu, Isao Yamamoto, Kimiko Nakagawa, Iwao Hasegawa, Shinji Akitomi, Daisuke Yajima, Tetsuo Ushiku, Hisako Saitoh, Tadaki Suzuki, Hirotaro Iwase
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引用次数: 0

Abstract

Minimally invasive autopsy (MIA) is an alternative to a full autopsy for the collection of tissue samples from patients' bodies using instruments such as a biopsy needle. MIA has been conducted in many cases of coronavirus disease 2019 (COVID-19) and has contributed to the elucidation of the disease pathogenesis. However, most cases analyzed are hospital deaths, and there are few reports on the application of MIA in out-of-hospital deaths with varying extents of post-mortem changes. In this study, MIA and autopsies were performed in 15 patients with COVID-19 2-30 days after death, including 11 out-of-hospital deaths. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome detection by reverse transcriptase quantitative polymerase chain reaction using MIA samples was mostly consistent with autopsy samples, particularly lung tissue, even in out-of-hospital cases. MIA had high sensitivity and specificity (> 0.80). Histological examination of lung tissue obtained by MIA showed characteristics of COVID-19 pneumonia, with 91% agreement with autopsy samples, whereas localization of SARS-CoV-2 protein in lung tissue was indicated by immunohistochemistry, with 75% agreement. In conclusion, these results suggest that MIA is applicable to out-of-hospital deaths due to COVID-19 with various postmortem changes, especially when autopsies are not available.

新冠肺炎对经微创尸检获得的组织样本进行分析,这些样本用于尸检后死亡的病理变性。
微创尸检(MIA)是使用活检针等仪器从患者体内采集组织样本的完整尸检的替代方案。MIA已在2019冠状病毒病(新冠肺炎)的许多病例中进行,并有助于阐明疾病的发病机制。然而,分析的大多数病例都是医院死亡,很少有关于MIA在院外死亡中应用的报告,这些死亡具有不同程度的死后变化。在这项研究中,15名新冠肺炎患者在死亡后2-30天进行了MIA和尸检,其中包括11例院外死亡。使用MIA样本通过逆转录酶定量聚合酶链式反应检测严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)基因组,与尸检样本,特别是肺组织,即使在医院外病例中,也基本一致。MIA具有高灵敏度和特异性(>0.80)。MIA获得的肺组织的组织学检查显示了新冠肺炎肺炎的特征,与尸检样本的一致性为91%,而免疫组织化学显示了SARS-CoV-2蛋白在肺组织中的定位,一致性为75%。总之,这些结果表明,MIA适用于新冠肺炎导致的具有各种死后变化的院外死亡,尤其是在无法进行尸检的情况下。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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