TSNAXIP1 is required for sperm head formation and male fertility.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2023-06-28 eCollection Date: 2023-01-01 DOI:10.1002/rmb2.12520

Tasrin Sultana, Tokuko Iwamori, Naoki Iwamori

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引用次数: 0

Abstract

Purpose: TRANSLIN (TSN) and its binding partner TSNAX have been reported to contribute to a wide spectrum of biological activities including spermatogenesis. TSN accompanies specific mRNA transport in male germ cells through intercellular bridges. A testis-expressed protein TSNAXIP1 was reported to interact with TSNAX. However the role of TSNAXIP1 in spermatogenesis remained unclear. This study aimed to elucidate the role of TSNAXIP1 in spermatogenesis and male fertility in mice.

Methods: TSNAXIP1 knockout (KO) mice were generated using the CRISPR-Cas9 system. The fertility, spermatogenesis, and sperm of TSNAXIP1 KO males were analyzed.

Results: TSNAXIP1, and especially its domains, are highly conserved between mouse and human. Tsnaxip1 was expressed in testis, but not in ovary. TSNAXIP1 KO mice were generated, and TSNAXIP1 KO males were found to be sub-fertile with smaller testis and lower sperm count. Although no overt abnormalities were observed during spermatogenesis, lack of TSNAXIP1 induced sperm head malformation, resulting in a unique flower-shaped sperm head. Moreover, abnormal anchorage of the sperm neck was frequently observed in TSNAXIP1 null sperm.

Conclusion: A testis-expressed gene TSNAXIP1 has important roles in sperm head morphogenesis and male fertility. Moreover, TSNAXIP1 could be a causative gene for human infertility.

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TSNAXIP1 是精子头部形成和男性生育能力所必需的。

目的：据报道，TRANSLIN（TSN）及其结合伙伴 TSNAX 有助于包括精子发生在内的多种生物活动。TSN通过细胞间桥伴随男性生殖细胞中特定的mRNA运输。据报道，一种睾丸表达蛋白 TSNAXIP1 与 TSNAX 相互作用。然而，TSNAXIP1在精子发生中的作用仍不清楚。本研究旨在阐明TSNAXIP1在小鼠精子发生和雄性生育能力中的作用：方法：利用 CRISPR-Cas9 系统生成 TSNAXIP1 基因敲除（KO）小鼠。方法：利用 CRISPR-Cas9 系统生成 TSNAXIP1 基因敲除（KO）小鼠，分析 TSNAXIP1 KO 雄性的生育能力、精子发生和精子：结果：TSNAXIP1，尤其是其结构域，在小鼠和人类之间高度保守。Tsnaxip1在睾丸中表达，但不在卵巢中表达。经研究发现，TSNAXIP1 KO小鼠的雄性睾丸较小，精子数量较少，属于弱精子症。虽然在精子发生过程中没有观察到明显的异常，但缺乏 TSNAXIP1 会导致精子头部畸形，形成独特的花形精子头部。此外，在TSNAXIP1无效精子中还经常观察到精子颈部的异常固定：结论：睾丸表达基因TSNAXIP1在精子头部形态发生和男性生育能力中具有重要作用。此外，TSNAXIP1 可能是人类不育症的致病基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.