Examining the inorganic elemental composition of lobster phyllosoma (Panulirus ornatus) with X-ray fluorescence microscopy.

IF 2.9 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metallomics Pub Date : 2023-06-01 DOI:10.1093/mtomcs/mfad038

Daniel R McDougall, Robert Deas, Daryl L Howard, Quinn P Fitzgibbon, Gregory G Smith, Andrew G Jeffs, Duncan J McGillivray

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Abstract

The ornate spiny rock lobster, Panulirus ornatus, is an attractive candidate for aquaculture. The larval stages of spiny lobsters, known as phyllosoma, are complex with many developmental stages. Very little is known about the inorganic element composition of phyllosoma. In this study, a novel method using synchrotron X-ray fluorescence microscopy (XFM) was applied to investigate the distributions of metals potassium (K), calcium (Ca), copper (Cu), zinc (Zn), the metalloid arsenic (As), and nonmetal bromine (Br) within individual phyllosoma at stages 3, 4, and 8 of their development. For the first time, 1 µm resolution synchrotron XFM images of whole phyllosoma as well as closer examinations of their eyes, mouths, setae, and tails were obtained. Elements accumulated in certain locations within phyllosoma, providing insight into their likely biological role for these organisms. This information may be useful for the application of dietary supplementation in the future to closed larval cycle lobster aquaculture operations.

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用 X 射线荧光显微镜观察龙虾鳃瘤（Panulirus ornatus）的无机元素组成。

华丽刺岩龙虾（Panulirus ornatus）是一种极具吸引力的水产养殖对象。刺龙虾的幼体阶段被称为藻体，发育阶段复杂。人们对藻体的无机元素组成知之甚少。本研究采用同步辐射 X 射线荧光显微镜（XFM）这一新颖的方法，研究了发育到第 3、4 和 8 阶段的单个叶瘤中金属钾（K）、钙（Ca）、铜（Cu）、锌（Zn）、类金属砷（As）和非金属溴（Br）的分布情况。研究人员首次获得了整个叶瘤的 1 µm 分辨率同步辐射 XFM 图像，并对叶瘤的眼睛、嘴巴、刚毛和尾巴进行了仔细观察。这些元素积聚在植食动物体内的某些位置，使人们得以深入了解它们在这些生物体内可能发挥的生物作用。这些信息可能有助于今后在封闭式幼虫周期龙虾养殖作业中应用膳食补充剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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