Evaluation of MicroRNA Expression Pattern (miR-28, miR-181a, miR-34a, and miR-31) in Patients with COVID-19 Admitted to ICU and Diabetic COVID-19 Patients.

IF 3.2 4区 医学 Q3 VIROLOGY
Intervirology Pub Date : 2023-01-01 Epub Date: 2023-03-07 DOI:10.1159/000529985
AliReza Khatami, Mohammad Taghizadieh, Javid Sadri Nahand, Mohammad Karimzadeh, Seyed Jalal Kiani, Khadijeh Khanaliha, Saeed Kalantari, Sara Chavoshpour, Hamed Mirzaei, Tahereh Donyavi, Farah Bokharaei-Salim
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引用次数: 0

Abstract

Introduction: MicroRNAs, or miRNAs, with regulatory performance in inflammatory responses and infection are the prevalent manifestations of severe coronavirus disease (COVID-19). This study aimed to evaluate whether PBMC miRNAs are diagnostic biomarkers to screen the ICU COVID-19 and diabetic COVID-19 subjects.

Methods: Candidate miRNAs were selected through previous studies, and then the PBMC levels of selected miRNAs (miR-28, miR-31, miR-34a, and miR-181a) were measured via quantitative reverse transcription PCR. The diagnostic value of miRNAs was determined by the receiver operating characteristic (ROC) curve. The bioinformatics analysis was utilized to predict the DEM genes and relevant bio-functions.

Results: The COVID-19 patients admitted to ICU had significantly greater levels of selected miRNAs compared to non-hospitalized COVID-19 and healthy people. Besides, the mean miR-28 and miR-34a expression levels in the diabetic COVID-19 group were significantly upregulated when compared with the non-diabetic COVID-19 group. ROC analyses demonstrated the role of miR-28, miR-34a, and miR-181a as new biomarkers to discriminate the non-hospitalized COVID-19 group from the COVID-19 patients admitted to ICU samples, and also miR-34a can probably act as a useful biomarker for screening diabetic COVID-19 patients. Using bioinformatics analyses, we found the performance of target transcripts in many bioprocesses and diverse metabolic routes such as the regulation of multiple inflammatory parameters.

Discussion: The difference in miRNA expression patterns between the studied groups suggested that miR-28, miR-34a, and miR-181a could be helpful as potent biomarkers for diagnosing and controlling COVID-19.

Abstract Image

Abstract Image

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评估入住重症监护室的 COVID-19 患者和糖尿病 COVID-19 患者的 MicroRNA 表达模式(miR-28、miR-181a、miR-34a 和 miR-31)。
导言:在炎症反应和感染中具有调控功能的微RNA(或称miRNA)是严重冠状病毒病(COVID-19)的普遍表现。本研究旨在评估 PBMC miRNAs 是否是筛选 ICU COVID-19 和糖尿病 COVID-19 受试者的诊断生物标志物:方法:通过以往的研究筛选出候选miRNA,然后通过反转录PCR定量检测所选miRNA(miR-28、miR-31、miR-34a和miR-181a)的PBMC水平。miRNA 的诊断价值由接收者操作特征曲线(ROC)决定。利用生物信息学分析预测DEM基因和相关生物功能:结果:与非住院的COVID-19患者和健康人相比,入住重症监护室的COVID-19患者所选miRNA的水平明显更高。此外,与非糖尿病 COVID-19 组相比,糖尿病 COVID-19 组的平均 miR-28 和 miR-34a 表达水平明显升高。ROC分析表明,miR-28、miR-34a和miR-181a是区分非住院COVID-19组和入住ICU样本的COVID-19患者的新生物标记物,而且miR-34a可能是筛查糖尿病COVID-19患者的有用生物标记物。通过生物信息学分析,我们发现了目标转录物在许多生物过程和多种代谢途径中的表现,如调节多种炎症参数:讨论:研究组之间 miRNA 表达模式的差异表明,miR-28、miR-34a 和 miR-181a 可作为诊断和控制 COVID-19 的有效生物标志物。
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来源期刊
Intervirology
Intervirology 医学-病毒学
CiteScore
5.40
自引率
0.00%
发文量
13
审稿时长
6-12 weeks
期刊介绍: ''Intervirology'' covers progress in both basic and clinical virus research, and aims to provide a forum for the various disciplines within virology. Issues publishing original papers alternate with thematic issues, focusing on clearly defined topics. This thematic concentration serves to make timely reviews, research reports and controversy easily accessible to both specialists in the field and those who want to keep track of the latest developments outside their own area of interest. In addition to original papers, regular issues publish short communications and letters to the editor to provide readers with a forum for the exchange of ideas and comments. The scope encompasses work on the molecular biology of human and animal viruses, including genome organization and regulation, and the structure and function of viral proteins. The pathogenesis, immunology, diagnosis, epidemiology, prophylaxis and therapy of viral diseases are considered.
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