Characterisation of changes in global genes expression in the lung of ICR mice in response to the inflammation and fibrosis induced by polystyrene nanoplastics inhalation.

IF 1.6 4区医学 Q4 TOXICOLOGY

Toxicological Research Pub Date : 2023-05-22 DOI:10.1007/s43188-023-00188-y

You Jeong Jin, Ji Eun Kim, Yu Jeong Roh, Hee Jin Song, Ayun Seol, Jumin Park, Yong Lim, Sungbaek Seo, Dae Youn Hwang

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引用次数: 1

Abstract

This study characterised the changes in global gene expression in the lung of ICR mice in response to the inflammation and fibrosis induced by the inhalation of 0.5 μm polystyrene (PS)-nanoplastics (NPs) at various concentrations (4, 8, and 16 μg/mL) for 2 weeks. The total RNA extracted from the lung tissue of NPs-inhaled mice was hybridised into oligonucleotide microarrays. Significant upregulation was detected in several inflammatory responses, including the number of immune cells in bronchoalveolar lavage fluid (BALF), the expression level of inflammatory cytokines, mucin secretion, and histopathological changes, while they accumulated average of 13.38 ± 1.0 μg/g in the lungs of the inhaled ICR mice. Similar responses were observed regarding the levels of fibrosis-related factors in the NPs-inhaled lung of ICR mice, such as pulmonary parenchymal area, expression of pro-fibrotic marker genes, and TGF-β1 downstream signalling without any significant hepatotoxicity and nephrotoxicity. In microarray analyses, 60 genes were upregulated, and 55 genes were downregulated in the lung of ICR mice during inflammation and fibrosis induced by NPs inhalation compared to the Vehicle-inhaled mice. Among these genes, many were categorised into several ontology categories, including the anatomical structure, binding, membrane, and metabolic process. Furthermore, the major genes in the upregulated categories included Igkv14-126000, Egr1, Scel, Lamb3, and Upk3b. In contrast, the major genes in the down-regulated categories were Olfr417, Olfr519, Rps16, Rap2b, and Vmn1r193. These results suggest several gene functional groups and individual genes as specific biomarkers respond to inflammation and fibrosis induced by PS-NPs inhalation in ICR mice.

Supplementary information: The online version contains supplementary material available at 10.1007/s43188-023-00188-y.

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ICR小鼠肺部对聚苯乙烯纳米塑料吸入诱导的炎症和纤维化反应的整体基因表达变化的特征。

本研究描述了ICR小鼠在吸入不同浓度（4、8和16μg/mL）的0.5μm聚苯乙烯（PS）-纳米塑料（NP）2周后，对炎症和纤维化反应时肺部整体基因表达的变化。将从吸入NPs的小鼠肺组织中提取的总RNA杂交成寡核苷酸微阵列。在几种炎症反应中检测到显著上调，包括支气管肺泡灌洗液（BALF）中免疫细胞的数量、炎性细胞因子的表达水平、粘蛋白分泌和组织病理学变化，而它们的平均累积值为13.38 ± μg/g。在ICR小鼠吸入的NPs肺中，观察到类似的纤维化相关因子水平，如肺实质面积、促纤维化标志物基因的表达和TGF-β1下游信号传导，而没有任何显著的肝毒性和肾毒性。在微阵列分析中，与载体吸入小鼠相比，在吸入NPs诱导的炎症和纤维化过程中，ICR小鼠的肺中有60个基因上调，55个基因下调。在这些基因中，许多被分为几个本体论类别，包括解剖结构、结合、膜和代谢过程。此外，上调类别中的主要基因包括Igkv14-126000、Egr1、Scel、Lamb3和Upk3b。相反，下调类别中的主要基因是Olfr417、Olfr519、Rps16、Rap2b和Vmn1r193。这些结果表明，在ICR小鼠中，几个基因功能组和单个基因作为特异性生物标志物对吸入PS NPs诱导的炎症和纤维化有反应。补充信息：在线版本包含补充材料，请访问10.1007/s43188-023-00188-y。

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来源期刊

Toxicological Research TOXICOLOGY-

CiteScore

4.20

自引率

4.30%

发文量

期刊介绍： Toxicological Research is the official journal of the Korean Society of Toxicology. The journal covers all areas of Toxicological Research of chemicals, drugs and environmental agents affecting human and animals, which in turn impact public health. The journal’s mission is to disseminate scientific and technical information on diverse areas of toxicological research. Contributions by toxicologists, molecular biologists, geneticists, biochemists, pharmacologists, clinical researchers and epidemiologists with a global view on public health through toxicological research are welcome. Emphasis will be given to articles providing an understanding of the toxicological mechanisms affecting animal, human and public health. In the case of research articles using natural extracts, detailed information with respect to the origin, extraction method, chemical profiles, and characterization of standard compounds to ensure the reproducible pharmacological activity should be provided.