Fusarium diversity associated with diseased cereals in China, with an updated phylogenomic assessment of the genus.

IF 14.1 1区 生物学 Q1 MYCOLOGY
Studies in Mycology Pub Date : 2023-07-01 Epub Date: 2023-02-22 DOI:10.3114/sim.2022.104.02
S L Han, M M Wang, Z Y Ma, M Raza, P Zhao, J M Liang, M Gao, Y J Li, J W Wang, D M Hu, L Cai
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引用次数: 0

Abstract

Fusarium species are important cereal pathogens that cause severe production losses to major cereal crops such as maize, rice, and wheat. However, the causal agents of Fusarium diseases on cereals have not been well documented because of the difficulty in species identification and the debates surrounding generic and species concepts. In this study, we used a citizen science initiative to investigate diseased cereal crops (maize, rice, wheat) from 250 locations, covering the major cereal-growing regions in China. A total of 2 020 Fusarium strains were isolated from 315 diseased samples. Employing multi-locus phylogeny and morphological features, the above strains were identified to 43 species, including eight novel species that are described in this paper. A world checklist of cereal-associated Fusarium species is provided, with 39 and 52 new records updated for the world and China, respectively. Notably, 56 % of samples collected in this study were observed to have co-infections of more than one Fusarium species, and the detailed associations are discussed. Following Koch's postulates, 18 species were first confirmed as pathogens of maize stalk rot in this study. Furthermore, a high-confidence species tree was constructed in this study based on 1 001 homologous loci of 228 assembled genomes (40 genomes were sequenced and provided in this study), which supported the "narrow" generic concept of Fusarium (= Gibberella). This study represents one of the most comprehensive surveys of cereal Fusarium diseases to date. It significantly improves our understanding of the global diversity and distribution of cereal-associated Fusarium species, as well as largely clarifies the phylogenetic relationships within the genus. Taxonomic novelties: New species: Fusarium erosum S.L. Han, M.M. Wang & L. Cai, Fusarium fecundum S.L. Han, M.M. Wang & L. Cai, Fusarium jinanense S.L. Han, M.M. Wang & L. Cai, Fusarium mianyangense S.L. Han, M.M. Wang & L. Cai, Fusarium nothincarnatum S.L. Han, M.M. Wang & L. Cai, Fusarium planum S.L. Han, M.M. Wang & L. Cai, Fusarium sanyaense S.L. Han, M.M. Wang & L. Cai, Fusarium weifangense S.L. Han, M.M. Wang & L. Cai. Citation: Han SL, Wang MM, Ma ZY, Raza M, Zhao P, Liang JM, Gao M, Li YJ, Wang JW, Hu DM, Cai L (2023). Fusarium diversity associated with diseased cereals in China, with an updated phylogenomic assessment of the genus. Studies in Mycology 104: 87-148. doi: 10.3114/sim.2022.104.02.

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与中国病害谷物相关的镰刀菌多样性,以及对该属的最新系统发生组学评估。
镰刀菌是重要的谷物病原体,对玉米、水稻和小麦等主要谷物作物造成严重的产量损失。然而,谷物上镰刀菌病害的病原菌还没有被很好地记录下来,原因是很难进行物种鉴定,而且围绕着属种和种的概念还存在争议。在本研究中,我们利用公民科学计划调查了中国主要谷物种植区 250 个地点的患病谷物作物(玉米、水稻和小麦)。从 315 个病害样本中共分离出 2 020 株镰刀菌。通过多焦点系统发育和形态特征,上述菌株被鉴定为 43 个种,其中包括本文描述的 8 个新种。本文提供了与谷物相关的镰刀菌种的世界清单,分别更新了世界和中国的 39 条和 52 条新记录。值得注意的是,在本研究中采集的样本中有 56% 同时感染了一种以上的镰刀菌,并对其详细的关联进行了讨论。根据科赫假说,本研究首次确认了 18 个菌种为玉米茎腐病的病原体。此外,本研究还根据 228 个组装基因组(本研究提供了 40 个基因组的测序结果)的 1 001 个同源位点构建了高置信度的物种树,支持镰刀菌(=吉伯菌)的 "狭义 "属概念。这项研究是迄今为止对谷物镰刀菌病害最全面的调查之一。它极大地提高了我们对谷物相关镰刀菌物种的全球多样性和分布的认识,并在很大程度上澄清了该属内部的系统发育关系。分类学上的新发现:新种:Fusarium erosum S.L. Han, M.M. Wang & L. Cai, Fusarium fecundum S.L. Han, M.M. Wang & L. Cai, Fusarium jinanense S.L. Han, M.M. Wang & L. Cai, Fusarium mianyangense S.L. Han, M.M. Wang & L. Cai, Fusarium notanense S.L. Han, M.M. Wang & L. Cai.Cai, Fusarium nothincarnatum S.L. Han, M.M. Wang & L. Cai, Fusarium planum S.L. Han, M.M. Wang & L. Cai, Fusarium sanyaense S.L. Han, M.M. Wang & L. Cai, Fusarium weifangense S.L. Han, M.M. Wang & L. Cai.引用:Han SL, Wang MM, Ma ZY, Raza M, Zhao P, Liang JM, Gao M, Li YJ, Wang JW, Hu DM, Cai L (2023).与中国病害谷物相关的镰刀菌多样性,以及对该属系统发生组的最新评估。Doi: 10.3114/sim.2022.104.02.
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来源期刊
Studies in Mycology
Studies in Mycology 生物-真菌学
CiteScore
35.60
自引率
3.00%
发文量
7
期刊介绍: The international journal Studies in Mycology focuses on advancing the understanding of filamentous fungi, yeasts, and various aspects of mycology. It publishes comprehensive systematic monographs as well as topical issues covering a wide range of subjects including biotechnology, ecology, molecular biology, pathology, and systematics. This Open-Access journal offers unrestricted access to its content. Each issue of Studies in Mycology consists of around 5 to 6 papers, either in the form of monographs or special focused topics. Unlike traditional length restrictions, the journal encourages submissions of manuscripts with a minimum of 50 A4 pages in print. This ensures a thorough exploration and presentation of the research findings, maximizing the depth of the published work.
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