Human pluripotent stem cell-derived extracellular vesicles: From now to the future.

IF 3.6 3区医学 Q3 CELL & TISSUE ENGINEERING

World journal of stem cells Pub Date : 2023-05-26 DOI:10.4252/wjsc.v15.i5.453

Bruno Moises de Matos, Marco Augusto Stimamiglio, Alejandro Correa, Anny Waloski Robert

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引用次数: 1

Abstract

Extracellular vesicles (EVs) are nanometric particles that enclose cell-derived bioactive molecules in a lipid bilayer and serve as intercellular communication tools. Accordingly, in various biological contexts, EVs are reported to engage in immune modulation, senescence, and cell proliferation and differentiation. Therefore, EVs could be key elements for potential off-the-shelf cell-free therapy. Little has been studied regarding EVs derived from human pluripotent stem cells (hPSC-EVs), even though hPSCs offer good opportunities for induction of tissue regeneration and unlimited proliferative ability. In this review article, we provide an overview of studies using hPSC-EVs, focusing on identifying the conditions in which the cells are cultivated for the isolation of EVs, how they are characterized, and applications already demonstrated. The topics reported in this article highlight the incipient status of the studies in the field and the significance of hPSC-EVs' prospective applications as PSC-derived cell-free therapy products.

Abstract Image

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人类多能干细胞衍生的细胞外囊泡:从现在到未来。

细胞外囊泡(EVs)是一种纳米颗粒，它将细胞来源的生物活性分子包裹在脂质双分子层中，并作为细胞间的通讯工具。因此，在各种生物学背景下，据报道，ev参与免疫调节、衰老、细胞增殖和分化。因此，电动汽车可能是潜在的现成无细胞治疗的关键因素。尽管人类多能干细胞提供了诱导组织再生和无限增殖能力的良好机会，但关于人类多能干细胞(hPSC-EVs)衍生的ev的研究很少。在这篇综述文章中，我们概述了使用hpsc - ev的研究，重点是确定用于分离ev的细胞培养条件，它们是如何表征的，以及已经证明的应用。本文报道的主题强调了该领域研究的初期状态以及hpsc - ev作为psc衍生的无细胞治疗产品的前景应用的意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of stem cells Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

7.80

自引率

4.90%

发文量

750

期刊介绍： The World Journal of Stem Cells (WJSC) is a leading academic journal devoted to reporting the latest, cutting-edge research progress and findings of basic research and clinical practice in the field of stem cells. It was launched on December 31, 2009 and is published monthly (12 issues annually) by BPG, the world''s leading professional clinical medical journal publishing company.