Cytotoxicity of dental self-curing resin for a temporary crown: an in vitro study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-11-01 Epub Date: 2023-04-26 DOI:10.12701/jyms.2023.00080

Jae-Wan Ko, Joon Sakong, Sohee Kang

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引用次数: 0

Abstract

Background: Residual monomer tests using high-performance liquid chromatography and cytotoxicity tests were performed to analyze the effect on the oral mucosa of a self-curing resin for provisional crown production.

Methods: A cytotoxicity test was performed to confirm whether leaked residual monomers directly affected oral mucosal cells. The cytotoxicity of the liquid and solid resin polymers was measured using a water-soluble tetrazolium (WST) test and microplate reader.

Results: In the WST assay using a microplate reader, 73.4% of the cells survived at a concentration of 0.2% liquid resin polymer. The cytotoxicity of the liquid resin polymer was low at ≤0.2%. For the solid resins, when 100% of the eluate was used from each specimen, the average cell viability was 91.3% for the solid resin polymer and 100% for the hand-mixed self-curing resin, which is higher than the cell viability standard of 70%. The cytotoxicity of the solid resin polymer was low.

Conclusion: Because the polymerization process of the self-curing resin may have harmful effects on the oral mucosa during the second and third stages, the solid resin should be manufactured indirectly using a dental model.

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临时牙冠用自固化树脂细胞毒性的体外研究。

背景:采用高效液相色谱法和细胞毒性试验分析了一种用于临时冠制作的自固化树脂对口腔黏膜的影响。方法:采用细胞毒性试验，确定泄漏的残留单体是否直接影响口腔粘膜细胞。采用水溶性四氮唑(WST)测试和微孔板读取器测定液体和固体树脂聚合物的细胞毒性。结果:在使用微孔板阅读器的WST实验中，在0.2%的液体树脂聚合物浓度下，73.4%的细胞存活。液体树脂聚合物的细胞毒性较低，≤0.2%。对于固体树脂，当每个样品使用100%的洗脱液时，固体树脂聚合物的平均细胞存活率为91.3%，手工混合自固化树脂的平均细胞存活率为100%，高于70%的细胞存活率标准。固体树脂聚合物的细胞毒性较低。结论:由于自固化树脂的聚合过程在第二和第三阶段可能对口腔黏膜产生有害影响，因此应采用牙体模型间接制备固体树脂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.