Neuroprotective Effect of Celastrus Paniculatus Seed Extract on Epilepsy and Epilepsy-associated Cognitive Deficits.

IF 1 Q4 NEUROSCIENCES

Basic and Clinical Neuroscience Pub Date : 2023-01-01 DOI:10.32598/bcn.2021.3154.1

Arti Ralta, Ajay Prakash, Praveen Kumar M, Rohit Kumar, Phulen Sarma, Alka Bhatia, Bikash Medhi, Amitava Chakrabarti

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引用次数: 0

Abstract

Introduction: Cognitive deficit is one of the common comorbidity accompanying epilepsy. The present study evaluated the effect of Celastrus paniculatus seed extract on seizure severity and cognitive deficit following the pentylenetetrazole (PTZ)-induced chemical kindling model.

Methods: PTZ kindling model was developed by daily administration of the sub-convulsive dose of PTZ 30 mg/kg for four weeks. After four weeks of induction, the following treatment, namely sodium valproic acid (SVA) 200 mg/kg, C. paniculatus 500 mg\kg, pergolide 2 mg/kg, C. paniculatus (250 mg\kg)+ Pergolide (1 mg/kg), and C. paniculatus (250 mg\kg)+ SVA (100 mg/kg) were administered 30 minutes prior to PTZ (30 mg/kg) injection for a period of next 14 days. Neurobehavioral parameters, including superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and dopamine levels were assessed and the Morris water maze test (MWM) and Grip strength test (GPS) were performed. Hematoxylin & Eosin (H&E) staining of hippocampal cornu ammonis (CA1), CA2, CA3, dentate gyrus (DG), and frontal cortex was performed.

Results: C. paniculatus (500 mg/kg) alone and in combination (C. paniculatus (250 mg\ kg)+ pergolide (1 mg/kg) and C. paniculatus (250 mg\kg)+ SVA (100 mg/kg)) significantly (P<0.05) reduced the seizure score, mean latency time, and distance traveled in the MWM. However, no significant effect was seen in GPS. Biochemical analysis showed elevated antioxidant markers, namely GSH, CAT, and SOD, and also elevated dopamine levels. C. paniculatus and its combination also significantly (P<0.05) protected against neuronal loss in the hippocampus and frontal cortex evidenced by H&E staining.

Conclusion: C. paniculatus alone and in combination with other agents may have the potential to treat epilepsy and associated cognitive deficits.

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南蛇藤种子提取物对癫痫和癫痫相关认知缺陷的神经保护作用。

引言：认知缺陷是癫痫常见的合并症之一。本研究评估了南蛇藤种子提取物对戊四唑（PTZ）诱导的化学点燃模型后癫痫发作严重程度和认知缺陷的影响。方法：PTZ点燃模型采用亚惊厥剂量PTZ 30mg/kg，连续4周。诱导四周后，在PTZ（30 mg/kg）注射前30分钟给予以下治疗，即丙戊酸钠（SVA）200 mg/kg、圆锥花菇500 mg/kg、藤架2 mg/kg、圆锥花菇（250 mg/kg）+藤架（1 mg/kg）和圆锥花菇（250 mg/kg）+SVA（100 mg/kg），持续14天。评估神经行为参数，包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和多巴胺水平，并进行Morris水迷宫测试（MWM）和握力测试（GPS）。对海马角（CA1）、CA2、CA3、齿状回（DG）和额叶皮层进行苏木精和曙红（H&E）染色。结果：单用和联合用药（单用和联用）（单用或联用）对癫痫及相关认知缺陷有治疗作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Basic and Clinical Neuroscience NEUROSCIENCES-

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

4 weeks

期刊介绍： BCN is an international multidisciplinary journal that publishes editorials, original full-length research articles, short communications, reviews, methodological papers, commentaries, perspectives and “news and reports” in the broad fields of developmental, molecular, cellular, system, computational, behavioral, cognitive, and clinical neuroscience. No area in the neural related sciences is excluded from consideration, although priority is given to studies that provide applied insights into the functioning of the nervous system. BCN aims to advance our understanding of organization and function of the nervous system in health and disease, thereby improving the diagnosis and treatment of neural-related disorders. Manuscripts submitted to BCN should describe novel results generated by experiments that were guided by clearly defined aims or hypotheses. BCN aims to provide serious ties in interdisciplinary communication, accessibility to a broad readership inside Iran and the region and also in all other international academic sites, effective peer review process, and independence from all possible non-scientific interests. BCN also tries to empower national, regional and international collaborative networks in the field of neuroscience in Iran, Middle East, Central Asia and North Africa and to be the voice of the Iranian and regional neuroscience community in the world of neuroscientists. In this way, the journal encourages submission of editorials, review papers, commentaries, methodological notes and perspectives that address this scope.