Canine parvovirus type 2 vaccines in Brazil: Viral load in commercial vaccine vials and phylogenetic analysis of the vaccine viruses

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-05-01 DOI:10.1016/j.biologicals.2023.101676

Michele Machado Lencina , Uwe Truyen , Weslei de Oliveira Santana , Diéssy Kipper , Ana Paula Longaray Delamare , Suelen Paesi , Vagner Ricardo Lunge , André Felipe Streck

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引用次数: 1

Abstract

Canine parvovirus type 2 (CPV-2) is the etiological agent of a highly contagious and frequently fatal disease in dogs. Live attenuated vaccines (LAV) are recommended to prevent and control this disease. Commercial vaccines are typically produced with CPV-2 strains adapted to cell culture and usually non-pathogenic. The present study aimed to determine the viral load of CPV-2 vaccines commercially available in Brazil and to characterize the vaccine virus by DNA analysis of its capsid gene. The results demonstrated that all vaccine strains presented high homology of the VP2 gene and they were all closely related to the original CPV-2 strains. However, vaccine strains presented several differences in comparison with field strains currently circulating in Brazil. Seventy-one vials contained viral loads ranging from 7.4E3 to 4.9E10 DNA copies/ml. Nine vials did not contain any detectable CPV-2 DNA. In conclusion, there are genetic and antigenic differences among CPV-2 vaccines and field strains. Additionally, some vaccines have been commercialized with low titers of CPV-2. It is important to improve the quality of the vaccines to prevent or reduce the spread of CPV-2 in Brazil.

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巴西犬细小病毒2型疫苗:商业疫苗瓶中的病毒载量和疫苗病毒的系统发育分析

犬细小病毒2型（CPV-2）是一种高度传染性且经常致命的犬疾病的病原体。推荐使用减毒活疫苗（LAV）预防和控制这种疾病。商业疫苗通常是用适应细胞培养的CPV-2菌株生产的，并且通常是非致病性的。本研究旨在确定巴西市场上可买到的CPV-2疫苗的病毒载量，并通过衣壳基因的DNA分析来表征疫苗病毒。结果表明，所有疫苗株均表现出VP2基因的高度同源性，且均与CPV-2原始株密切相关。然而，与目前在巴西流行的现场菌株相比，疫苗菌株存在一些差异。71个小瓶含有7.4E3至4.9E10 DNA拷贝/ml的病毒载量。九个小瓶不含任何可检测的CPV-2 DNA。总之，CPV-2疫苗和现场菌株之间存在遗传和抗原差异。此外，一些疫苗已经商业化，具有低滴度的CPV-2。提高疫苗质量对于预防或减少CPV-2在巴西的传播非常重要。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.