The hepatoprotective effect of Sophora viciifolia fruit extract against acetaminophen-induced liver injury in mice.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of applied biomedicine Pub Date : 2023-06-01 DOI:10.32725/jab.2023.008

Shanshan Qi, Beibei Lin, Sanqiao Wu, Hao Hao, Hongxin Zheng, Xiang Liu, Xiaoying Zhang, Lijuan Yue, Chen Chen

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Abstract

This research demonstrated the protective effect and possible mechanism of the Sophora viciifolia extract (SVE) against acetaminophen-induced liver injury in mice. The levels of ALT and AST in the serum and antioxidant enzyme activity in the liver were measured. We used immunohistochemistry to detect CYP2E1, Nrf2, and Keap1 protein expression in the liver. The mRNA expression in the liver of TNF-α, NF-κB, and IL-6, Nrf2 and its downstream genes HO-1 and GCLC were measured by qRT-PCR. We found that SVE could decrease the ALT and AST levels, promote the activities of SOD, CAT, GSH-Px, and GSH, and ameliorate pathological liver lesions. SVE could down-regulate the mRNA expression of inflammatory factors and up-regulate Nrf2, HO-1 and GCLC. SVE reduced the protein expression of the CYP2E1 and increased the Nrf2 and Keap1. SVE has been shown to have a protective effect against APAP-induced liver injury, possibly through activation of the Keap1-Nrf2 pathway.

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苦参果提取物对对乙酰氨基酚所致小鼠肝损伤的保护作用。

本研究探讨了苦参提取物(SVE)对对乙酰氨基酚所致小鼠肝损伤的保护作用及其可能机制。测定血清ALT、AST水平及肝脏抗氧化酶活性。我们采用免疫组化方法检测肝脏中CYP2E1、Nrf2和Keap1蛋白的表达。采用qRT-PCR检测肝脏组织中TNF-α、NF-κB、IL-6、Nrf2及其下游基因HO-1、GCLC mRNA的表达。我们发现SVE可以降低ALT和AST水平，提高SOD、CAT、GSH- px和GSH的活性，改善病理性肝脏病变。SVE可下调炎性因子mRNA表达，上调Nrf2、HO-1和GCLC表达。SVE降低CYP2E1蛋白表达，增加Nrf2和Keap1蛋白表达。SVE已被证明对apap诱导的肝损伤具有保护作用，可能是通过激活Keap1-Nrf2通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.