A module centered on the transcription factor Msn2 from arbuscular mycorrhizal fungus Rhizophagus irregularis regulates drought stress tolerance in the host plant

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2023-06-27 DOI:10.1111/nph.19077

Xiaoning Fan, Hongyun Xie, Xinru Huang, Shuyuan Zhang, Yuying Nie, Hui Chen, Xianan Xie, Ming Tang

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引用次数: 1

Abstract

Arbuscular mycorrhizal (AM) fungi can form mutualistic endosymbiosis with > 70% of land plants for obtaining fatty acids and sugars, in return, AM fungi promote plant nutrients and water acquisition to enhance plant fitness. However, how AM fungi orchestrate its own signaling components in response to drought stress remains elusive.
Here, we identify a transcription factor containing C2H2 zinc finger domains, RiMsn2 from Rhizophagus irregularis. To characterize the RiMsn2, we combined heterologous expression, subcellular localization in yeasts, and biochemical and molecular studies with reverse genetics approaches during the in planta phase.
The results indicate that RiMsn2 is highly conserved across AM fungal species and induced during the early stages of symbiosis. It is significantly upregulated in mycorrhizal roots under severe drought conditions. The nucleus-localized RiMsn2 regulates osmotic homeostasis and trehalose contents of yeasts. Importantly, gene silencing analyses indicate that RiMsn2 is essential for arbuscule formation and enhances plant tolerance to drought stress. Results from yeasts and biochemical experiments suggest that the RiHog1-RiMsn2-STREs module controls the drought stress-responsive genes in AM fungal symbiont.
In conclusion, our findings reveal that a module centered on the transcriptional activator RiMsn2 from AM fungus regulates drought stress tolerance in host plant.

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以丛枝菌根真菌Rhizophagus irregularis转录因子Msn2为中心的模块调节寄主植物的抗旱性

丛枝菌根（AM）真菌可以与>； 70%的陆地植物获得脂肪酸和糖，作为回报，AM真菌促进植物营养和水分获取，以增强植物适应性。然而，AM真菌如何协调自身的信号成分来应对干旱胁迫仍然难以捉摸。在这里，我们鉴定了一种含有C2H2锌指结构域的转录因子，RiMsn2，来自Rhizophagus irregularis。为了表征RiMsn2，我们将异源表达、酵母亚细胞定位、生化和分子研究与植物内阶段的反向遗传学方法相结合。结果表明，RiMsn2在AM真菌物种中高度保守，并在共生的早期阶段被诱导。在严重干旱条件下，它在菌根根中显著上调。细胞核定位的RiMsn2调节酵母的渗透稳态和海藻糖含量。重要的是，基因沉默分析表明，RiMsn2对熊果苷的形成至关重要，并增强了植物对干旱胁迫的耐受性。酵母和生物化学实验的结果表明，RiHog1-RiMsn2-STREs模块控制AM真菌共生体中的干旱胁迫响应基因。总之，我们的研究结果表明，一个以AM真菌转录激活因子RiMsn2为中心的模块调节寄主植物的干旱胁迫耐受性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.