Simultaneous quantitation of nine carbazole alkaloids from Murraya koenigii (L.) Spreng by 1H qNMR spectroscopy

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2024-09-16 DOI:10.1080/14786419.2023.2219819

引用次数: 0

Abstract

Murraya koenigii leaves are widely used as a spice and also have several biological activities. The major active constituents are carbazole alkaloids. Quantitation by HPLC or HPTLC requires pure marker compounds, whereas nuclear magnetic resonance spectroscopy can be used as a quantitative technique without the requirement of a pure marker compound. An alkaloid-rich fraction was prepared from the leaves and a validated qNMR method was developed for the quantitation of nine carbazole alkaloids, namely mahanimbine, girinimbine, koenimbine, koenine, kurrayam, mukonicine, isomahanimbine, euchristine B and bismahanine. One of the major compounds, koenimbine, was isolated and quantified by HPTLC to compare the results. The results obtained by qNMR were compared with the reported yields of these compounds.

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利用 1H qNMR 光谱同时定量 Murraya koenigii (L.) Spreng 中的九种咔唑生物碱。

墨角兰叶被广泛用作香料，同时还具有多种生物活性。其主要活性成分是咔唑生物碱。通过 HPLC 或 HPTLC 进行定量需要纯标记化合物，而核磁共振波谱可用作定量技术，无需纯标记化合物。从叶片中制备了富含生物碱的馏分，并开发了一种有效的 qNMR 方法，用于定量分析九种咔唑生物碱，即 mahanimbine、girinimbine、koenimbine、koenine、kurrayam、mukonicine、isomahanimbine、euchristine B 和 bismahanine。对其中一种主要化合物柯宁宾进行了分离，并通过 HPTLC 进行了定量，以比较结果。通过 qNMR 获得的结果与报告的这些化合物的产量进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.