Erratum.

Abstract

FIGURE 5. Axonal (Wallerian) degeneration in adult human sural nerve. (Wallerian) degeneration in adult human sural nerve. Loss of neurofilament staining of larger axons is apparent within areas of myelin basic protein (MBP)-stained myelin. (A) Toluidine blue-stained plastic sections show myelin ovoids and irregularly shaped myelin sheaths. Scattered Schwann cells associated with myelin and other endoneurial cells contain lipid debris or droplets. Bar = 10 μM. (B) Neurofilament-stained frozen sections show round pale regions (arrow at the top right of image) that likely represent the loss of larger axons within areas of residual myelin. Smaller axons are relatively preserved. Bar = 20 μM. (C) Ultrastructural analysis shows a damaged axon (left) with irregular cytoplasm inside thick myelin associated with surrounding Schwann cell cytoplasm containing myelin debris and lipid droplets (arrow left). An endoneurial macrophage (right) (not likely a Schwann cell as it has no surrounding basal lamina) contains myelin debris and lipid droplets (arrow right). Bars = 2 μM. (D) Acid phosphatase (AcP) stains scattered endoneurial histiocytes (red). Bar = 50 μM. (E) Neural cell adhesion molecule (NCAM) (green) stains non-myelinating Schwann cells (see I). P0 protein (P0) (red) stains myelin sheaths (see H). There is minimal overlap (yellow). (F) NCAM and MBP show overlap (yellow) on both NCAM cells and some MBP-containing myelin. (G) Many MBP myelin regions have central regions with no neurofilament-stained axons (arrow). (H) Scattered P0 regions have central regions with no neurofilament-stained axons (arrow). (I) Most NCAM (red) regions have associated co-stained axons (yellow) suggesting acute axon loss is less prominent among small-sized axons. Bars for E–I = 100 μM. DOI: 10.1111/nan.12906