Exploring the Associations and Molecular Impacts of miR-146a/rs2910164 and miR-196a2/rs185070757 with Rheumatoid Arthritis in a Pakistani Population.

IF 2 4区医学 Q3 GENETICS & HEREDITY

Lifestyle Genomics Pub Date : 2023-01-01 Epub Date: 2023-06-27 DOI:10.1159/000526937

Yasir Ali, Aamir Khan, Mehran Akhtar, Suleman Khan, Zia Ul Islam, Nadia Farooqi, Aftab Ali Shah, Yangchao Chen, Fazal Jalil

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引用次数: 0

Abstract

Introduction: MicroRNAs (miRNAs) are a new class of molecules that participate in post-transcriptional regulation of gene expression and hence have been reported to have a crucial role in the pathogenesis of rheumatoid arthritis (RA). We aimed to investigate the association of miR-146a rs2910164 (G/C) and miR-196a2 rs185070757 (T/G) with RA susceptibility in Pakistani patients and to bioinformatically predict the molecular function of these miRNAs.

Methods: A case-control study on 600 individuals was conducted, including 300 RA patients and 300 matching healthy controls. Genotyping was performed by tetra-primer amplification of refractory mutation system-polymerase chain reaction, and the association between variants and RA was statistically determined using different models.

Results: For the variant rs2910164 (G/C) in miR-146a, no difference in genotype distribution was observed between RA cases and controls, as determined using co-dominant (χ2 = 4.33; p = 0.114), homozygous dominant (C/C vs. G/G + C/G) (OR = 0.740 [0.531-1.032]; p = 0.091), homozygous recessive (G/G vs. C/C + G/C) (odds ratio [OR] = 01.432 [0.930-2.206]; p = 0.126), heterozygous (G/C vs. C/C + G/G) (OR = 1.084 [0.786-1.494]; p = 0.682), and additive (OR 0.778 [0.617-0.981]; p = 0.039) models. Similarly, the GT genotype in the rs185070757 (T/G) miR-196a2 variant did not differ between cases and controls with any models (p > 0.05). For the first time, we report no association of miR-146a rs2910164 (G/C) and miR-196a2 rs185070757 (T/G) with RA in a Pakistani population. A subsequent bioinformatic analysis revealed that the CC genotype of miR-146a rs2910164 might have a protective role against RA pathogenesis, with no effect observed with the miR-196a2 rs185070757.

Conclusion: Our findings suggest that these miRNAs might have little-to-no impact on the RA pathogenesis in the Pakistani population.

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在巴基斯坦人群中探讨miR-146a/rs2910164和miR-196a/rs185070757与类风湿性关节炎的相关性和分子影响。

引言：微小RNA（miRNA）是一类参与基因表达转录后调控的新分子，因此已被报道在类风湿性关节炎（RA）的发病机制中发挥着至关重要的作用。我们旨在研究miR-146a rs2910164（G/C）和miR-196a2 rs185070757（T/G）与巴基斯坦患者RA易感性的关系，并从生物信息学角度预测这些miRNA的分子功能。通过难治性突变系统聚合酶链式反应的四引物扩增进行基因分型，并使用不同的模型统计确定变异与RA之间的关联。结果：对于miR-146a中的变异体rs2910164（G/C），RA病例和对照组之间没有观察到基因型分布的差异，这是通过共显性（χ2=4.33；p=0.114）、纯合显性（C/C vs.G/G+C/G）（OR=0.740[0.531-1.032]；p=0.091）、纯合子隐性（G/G vs.C/C+G/C）（比值比[OR]=0.1432[0.930-2.26]；p=0.126）、杂合（G/C vs。C/C+G/G）（OR=1.084[0.76-1494]；p=0.682）和加性（OR0.778[0.617-0.981]；p=0.039）模型。同样，rs185070757（T/G）miR-196a2变体中的GT基因型在病例和任何模型的对照组之间都没有差异（p＞0.05）。我们首次报道了在巴基斯坦人群中miR-146a rs2910164（G/C）和miR-196a2rs185070755（T/G）与RA没有关联。随后的生物信息学分析显示，miR-146a rs2910164的CC基因型可能对RA的发病机制具有保护作用，而miR-196a2 rs185070757没有观察到影响。结论：我们的研究结果表明，这些miRNA可能对巴基斯坦人群的RA发病机制几乎没有影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lifestyle Genomics Agricultural and Biological Sciences-Food Science

CiteScore

4.00

自引率

7.70%

发文量

审稿时长

28 weeks

期刊介绍： Lifestyle Genomics aims to provide a forum for highlighting new advances in the broad area of lifestyle-gene interactions and their influence on health and disease. The journal welcomes novel contributions that investigate how genetics may influence a person’s response to lifestyle factors, such as diet and nutrition, natural health products, physical activity, and sleep, amongst others. Additionally, contributions examining how lifestyle factors influence the expression/abundance of genes, proteins and metabolites in cell and animal models as well as in humans are also of interest. The journal will publish high-quality original research papers, brief research communications, reviews outlining timely advances in the field, and brief research methods pertaining to lifestyle genomics. It will also include a unique section under the heading “Market Place” presenting articles of companies active in the area of lifestyle genomics. Research articles will undergo rigorous scientific as well as statistical/bioinformatic review to ensure excellence.