Therapeutic effect of C-type natriuretic peptide on persistent pain in a rat knee arthritis model.

IF 1.3 4区 医学 Q3 ORTHOPEDICS
Shoichi Hasegawa, Jae-Sung An, Jun Hino, Yusuke Amano, Yusuke Nakagawa, Kazumasa Miyatake, Hiroki Katagiri, Tomomasa Nakamura, Ichiro Sekiya, Hideyuki Koga, Kunikazu Tsuji
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Abstract

Background: Intra-articular injection of C-type natriuretic peptide (CNP) at the acute inflammatory stage suppressed fibrotic changes in the infrapatellar fat pad (IFP), articular cartilage degeneration, and persistent pain in a monoiodoacetic acid (MIA)-induced rat knee arthritis model. In this study, we administered CNP during the inflammation subsiding period to evaluate CNP effectiveness in knees with osteoarthritis (OA) pathology.

Methods: 20 male Wistar rats were randomly divided into two groups. The rats received an intra-articular injection of MIA solution in the right knee to induce inflammation-induced joint degeneration. One group subsequently received an intra-articular CNP injection for six consecutive days from day 8, whereas another group received vehicle solution. Pain avoidance behavior tests and histological analyses were conducted to examine the therapeutic effects of CNP.

Results: The incapacitance test indicated that the percent weight on the ipsilateral limb decreased after MIA injection by day 4 and continued to decrease until the end of the experiment in the vehicle group, suggesting persistent pain in the knee. Intra-articular injection of CNP reversed the weight-bearing ratio on day 19. Histological evaluation showed that the CNP group had more residual fat tissue in the IFP and fewer calcitonin gene-related peptide-positive nerve endings compared to the vehicle group. CNP could not reverse articular cartilage degeneration.

Conclusions: Intra-articular injection of CNP after the IFP fibrosis onset had no significant effect on OA severity and extent. Nevertheless, CNP might be utilized therapeutically for OA treatment since it can alleviate persistent knee pain and inhibit structural changes in residual fat tissue.

c型利钠肽对大鼠膝关节关节炎模型持续性疼痛的治疗作用。
背景:在单碘乙酸(MIA)诱导的大鼠膝关节关节炎模型中,急性炎症期关节内注射c型利钠肽(CNP)可抑制髌下脂肪垫(IFP)的纤维化改变、关节软骨退变和持续疼痛。在这项研究中,我们在炎症消退期间给予CNP,以评估CNP对骨性关节炎(OA)病理膝关节的有效性。方法:20只雄性Wistar大鼠随机分为两组。大鼠右膝关节内注射MIA溶液,诱导炎症性关节变性。一组从第8天开始连续6天关节内注射CNP,另一组连续6天注射载药溶液。通过疼痛回避行为测试和组织学分析来检验CNP的治疗效果。结果:失能试验显示,注射MIA后第4天同侧肢体重量百分比下降,并持续下降至实验结束,提示膝关节持续疼痛。第19天关节内注射CNP逆转负重比。组织学评价显示,与载药组相比,CNP组IFP中残余脂肪组织较多,降钙素基因相关肽阳性神经末梢较少。CNP不能逆转关节软骨退变。结论:IFP纤维化发生后关节内注射CNP对骨性关节炎的严重程度和程度无显著影响。然而,CNP可能用于OA治疗,因为它可以减轻持续的膝关节疼痛和抑制残余脂肪组织的结构变化。
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来源期刊
Journal of Orthopaedic Surgery
Journal of Orthopaedic Surgery ORTHOPEDICS-SURGERY
CiteScore
3.10
自引率
0.00%
发文量
91
审稿时长
13 weeks
期刊介绍: Journal of Orthopaedic Surgery is an open access peer-reviewed journal publishing original reviews and research articles on all aspects of orthopaedic surgery. It is the official journal of the Asia Pacific Orthopaedic Association. The journal welcomes and will publish materials of a diverse nature, from basic science research to clinical trials and surgical techniques. The journal encourages contributions from all parts of the world, but special emphasis is given to research of particular relevance to the Asia Pacific region.
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