Effect of Karas (Aquilaria malaccensis) on Male Reproductive Organs and Sperm Quality in Adult Sprague Dawley Rats.

IF 1.1 Q3 BIOLOGY

Tropical life sciences research Pub Date : 2023-03-01 Epub Date: 2023-03-31 DOI:10.21315/tlsr2023.34.1.13

Norahidah Zaidi, Mohd Nizam Haron, Connie Fay Komilus, Fathurrahman Lananan, Ha Hou Chew, Nadzifah Yaakub, Asmad Kari

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Abstract

Reproductive health and male fertility are closely related to dietary practices. In recent years, Malaysia has shown a lot of interest in using herbal plants as dietary supplements or in the treatment of numerous diseases. Aquilaria malaccensis, commonly known as karas or gaharu, has recently gained attention for its potential to cure many diseases due to its pharmacological properties. However, studies on its effect on male fertility and reproductive organs are very scarce. This study was conducted to determine the effect of A. malaccensis on male reproductive organs' weight (testis, epididymis, prostate gland and seminal vesicle) and sperm quality (sperm count, sperm morphology and sperm motility) in adult Sprague Dawley rats. Twenty-four male Sprague Dawley rats were allocated into four treatment groups; Control (C: 1 mL of distilled water, n = 6), Treatment 1 (T1: 1 g A. malaccensis/kg body weight, n = 6), Treatment 2 (T2: 2 g A. malaccensis/kg body weight, n = 6) and Treatment 3 (T3: 3 g A. malaccensis/kg body weight, n = 6), respectively. Distilled water and A. malaccensis were administered by oral gavage once daily for 28 days. The rats were euthanised on Day 29 for assessment of reproductive organs' weight and sperm quality. Result shows that weight of testis, epididymis, prostate gland, seminal vesicle and sperm motility did not differ (p > 0.05) among control and treated groups. A significant increase (p < 0.05) of sperm number (1.36 × 10^-6) and a decrease (p < 0.05) in percentage of the abnormal sperm (8.17%) were observed in T1 group when compared to Control group. Incremental dosage of A. malaccensis seemed to decrease number of sperm (T3: 0.78 × 10^-6 < T1: 1.36 × 10^-6 with p < 0.05) and increase percentage of abnormal sperm (T3: 18.83% > T2: 12.17% > T1: 8.17% with p < 0.05). In conclusion, the administration of either 1, 2 or 3 grams of A. malaccensis did not alter the reproductive organs' weight and sperm motility. However, the higher concentration of A. malaccensis consumed by the rats seemed to have detrimental effects on the number and morphology of sperm.

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卡拉丝（Aquilaria malaccensis）对成年 Sprague Dawley 大鼠雄性生殖器官和精子质量的影响

生殖健康和男性生育能力与饮食习惯密切相关。近年来，马来西亚对使用草本植物作为膳食补充剂或治疗多种疾病表现出浓厚的兴趣。Aquilaria malaccensis（俗称 karas 或 gaharu）因其药理特性而具有治疗多种疾病的潜力，近来备受关注。然而，有关其对男性生育能力和生殖器官影响的研究却非常少。本研究旨在确定 A. malaccensis 对成年 Sprague Dawley 大鼠雄性生殖器官重量（睾丸、附睾、前列腺和精囊）和精子质量（精子数量、精子形态和精子活力）的影响。将 24 只雄性 Sprague Dawley 大鼠分为四个处理组：对照组（C：1 mL 蒸馏水，n = 6）、处理 1 组（T1：1 g A. malaccensis/kg体重，n = 6）、处理 2 组（T2：2 g A. malaccensis/kg体重，n = 6）和处理 3 组（T3：3 g A. malaccensis/kg体重，n = 6）。蒸馏水和孔雀石绿通过口腔灌胃给药，每天一次，连续 28 天。第 29 天对大鼠实施安乐死，以评估生殖器官的重量和精子质量。结果显示，对照组和处理组的睾丸、附睾、前列腺、精囊重量和精子活力没有差异（p > 0.05）。与对照组相比，T1 组的精子数量（1.36 × 10-6）明显增加（p < 0.05），畸形精子百分比（8.17%）下降（p < 0.05）。A.malaccensis剂量的增加似乎减少了精子数量（T3：0.78 × 10-6 < T1：1.36 × 10-6，p < 0.05），增加了畸形精子的百分比（T3：18.83% > T2：12.17% > T1：8.17%，p < 0.05）。总之，服用 1、2 或 3 克 A. malaccensis 不会改变生殖器官的重量和精子活力。然而，大鼠摄入较高浓度的孔雀石绿似乎会对精子的数量和形态产生不利影响。

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来源期刊

Tropical life sciences research BIOLOGY-

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.