Nitrate Reductase Assay for Rapid Determination of Methicillin-Resistant Staphylococcus aureus Clinical Isolates.

Laboratory medicine Pub Date : 2024-03-07 DOI:10.1093/labmed/lmad056

Kubra Yildirim, Cemilenur Atas, Esra Tanyel Akcit, Majda Licina, Ozlem Koyuncu Ozyurt, Mehmet Akif Gun, Ece Simsek, Ahmet Yilmaz Coban

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Abstract

Objective: To evaluate the performance of nitrate reductase assay (NRA), a rapid, colorimetric method for the determination of methicillin resistance in Staphylococcus aureus isolates obtained from the culture collection of the Akdeniz University Hospital Central Laboratory, Antalya, Türkiye.

Materials and methods: Identification for all 290 S aureus isolates at the species level was performed via matrix-assisted laser desorption/ionization-time of flight. Isolates were tested with NRA for methicillin resistance. The cefoxitin broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute was used as the reference method in the study. S aureus ATCC 29213 and S aureus ATCC 43300 strains were used for quality control.

Results: According to Food and Drug Administration criteria, the category agreement between NRA and BMD was found to be 100%. The essential agreement between both methods was determined to be 96.20%. There is no minor, major, or extremely major discrepancy between both methods.

Conclusion: The results show that NRA is a rapid, practical, and reliable colorimetric method for detecting MRSA.

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用于快速测定耐甲氧西林金黄色葡萄球菌临床分离物的硝酸还原酶测定法。

目的：评估硝酸还原酶测定法（NRA）的性能：评估硝酸还原酶测定法（NRA）的性能，该方法是一种快速比色法，用于测定从土耳其安塔利亚阿克丹尼兹大学医院中央实验室培养物中分离的金黄色葡萄球菌对甲氧西林的耐药性：通过基质辅助激光解吸/电离飞行时间法对所有 290 株金黄色葡萄球菌分离物进行了物种鉴定。用 NRA 对分离株进行了甲氧西林耐药性检测。本研究采用临床与实验室标准研究所推荐的头孢西丁肉汤微量稀释法（BMD）作为参考方法。金黄色葡萄球菌 ATCC 29213 株和金黄色葡萄球菌 ATCC 43300 株用于质量控制：根据食品和药物管理局的标准，NRA 和 BMD 的分类一致性为 100%。两种方法的基本一致率为 96.20%。结论：结果表明，NRA 是一种快速、准确的检测方法：结果表明，NRA 是一种快速、实用、可靠的检测 MRSA 的比色法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Laboratory medicine

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