Metagenomic and metatranscriptomic exploration of the Egyptian Red Sea sponge Theonella sp. associated microbial community

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-08-01 DOI:10.1016/j.margen.2023.101032

Manar El Samak , Samira Zakeer , Amro Hanora , Samar M. Solyman

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引用次数: 2

Abstract

Marine sponges associated microorganisms are considered to be prolific source of bioactive natural products. Omics-based techniques such as metagenomics and metatranscriptomics have been used as effective tools to discover natural products. In this study, we used integrated metagenomic and metatranscriptomic analysis of three samples of the Egyptian Red Sea sponge Theonella sp. microbiome to obtain a complete picture of its biosynthetic activity to produce bioactive compounds. Our data revealed high biodiversity of the Egyptian sponge microbiota represented by 38 bacterial phyla with Candidate Phylum Poribacteria as the most abundant phyla with an average of 27.5% of the microbial community. The analysis also revealed high biosynthetic activity of the sponge microbiome through detecting different types of biosynthetic gene clusters (BGCs) with predicted antibacterial, cytotoxic and inhibitory bioactivity and the majority of these clusters were found to be actively transcribed. The complete BGCs of the cytotoxic theonellamide and misakinolide were detected and found to be actively transcribed. The majority of the detected BGCs were predicted to be novel as they did not show any similarity with any known cluster in the MIBiG database.

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埃及红海海绵Theonella sp.相关微生物群落的宏基因组和宏转录组研究

与海绵相关的微生物被认为是生物活性天然产物的丰富来源。基于奥密克戎的技术，如宏基因组学和宏转录组学，已被用作发现天然产物的有效工具。在这项研究中，我们对埃及红海海绵Theonella sp.的三个样本进行了综合宏基因组和宏转录组分析，以获得其产生生物活性化合物的生物合成活性的全貌。我们的数据显示，以38个细菌门为代表的埃及海绵微生物群具有高度的生物多样性，候选Poribacteria门是最丰富的门，平均占微生物群落的27.5%。分析还通过检测具有预测的抗菌、细胞毒性和抑制生物活性的不同类型的生物合成基因簇（BGCs），揭示了海绵微生物组的高生物合成活性，并且发现这些簇中的大多数是主动转录的。检测到具有细胞毒性的席内酰胺和米沙酮内酯的完整BGC，并发现其被活性转录。大多数检测到的BGC被预测为新颖的，因为它们与MIBiG数据库中的任何已知聚类都没有显示出任何相似性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.