Decellularized Extracellular Matrix for Tissue Engineering (Review).

IF 1.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
E V Isaeva, E E Beketov, N V Arguchinskaya, S А Ivanov, P V Shegay, А D Kaprin
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引用次数: 3

Abstract

In recent years, decellularized tissues have evolved into a new, full-fledged platform for the creation of tissue-engineered constructions. Extracellular matrix (ECM) of each tissue provides a unique tissue-specific microenvironment for resident cells with the structure and biochemical signaling required for their functioning. The decellularized ECM (dECM) has been established to influence cell differentiation. The review provides recent data on the composition and functions of the ECM, methods for obtaining decellularized tissues, and their application in tissue engineering depending on their physical form (scaffold, powder, or hydrogel). The effect of the matrix source, decellularization and sterilization techniques on dECM composition has been considered. Regulatory mechanisms of cell differentiation by the extracellular matrix are discussed. Differences in the protein composition of the native and decellularized materials are presented. Application of dECM in the bioink composition for regeneration of various tissues using bioprinting technologies is also considered. It has been concluded that successful application of dECM in tissue engineering and regenerative medicine requires a permanent and biologically suitable dECM source, optimized tissue decellularization protocols, improved mechanical properties of dECM-derived bioinks, and prevention of immunological reaction of the organism.

组织工程脱细胞细胞外基质(综述)。
近年来,脱细胞组织已经发展成为一个新的、成熟的平台,用于创建组织工程结构。每个组织的细胞外基质(ECM)为驻留细胞提供了独特的组织特异性微环境,具有其功能所需的结构和生化信号。脱细胞ECM (dECM)已被证实对细胞分化有影响。这篇综述提供了ECM的组成和功能、获得脱细胞组织的方法以及它们在组织工程中的应用(取决于它们的物理形式(支架、粉末或水凝胶))的最新数据。考虑了基质来源、脱细胞和灭菌技术对dECM组成的影响。讨论了细胞外基质对细胞分化的调控机制。在天然和脱细胞材料的蛋白质组成的差异提出。还考虑了dECM在生物打印技术中用于各种组织再生的生物墨水组合物中的应用。因此,dECM在组织工程和再生医学中的成功应用需要一个永久的和生物学上合适的dECM来源,优化的组织脱细胞方案,提高dECM衍生生物墨水的力学性能,以及防止生物体的免疫反应。
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来源期刊
Sovremennye Tehnologii v Medicine
Sovremennye Tehnologii v Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
1.80
自引率
0.00%
发文量
38
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