Human in vitro blood barrier models: architectures and applications.

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-06-20 DOI:10.1080/21688370.2023.2222628
Brittany E Watson, Julia A Miles, Melissa A Moss
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引用次数: 0

Abstract

Blood barriers serve as key points of transport for essential molecules as well as lines of defense to protect against toxins. In vitro modeling of these barriers is common practice in the study of their physiology and related diseases. This review describes a common method of using an adaptable, low cost, semipermeable, suspended membrane to experimentally model three blood barriers in the human body: the blood-brain barrier (BBB), the gut-blood barrier (GBB), and the air-blood barrier (ABB). The GBB and ABB both protect from the outside environment, while the BBB protects the central nervous system from potential neurotoxic agents in the blood. These barriers share several commonalities, including the formation of tight junctions, polarized cellular monolayers, and circulatory system contact. Cell architectures used to mimic barrier anatomy as well as applications to study function, dysfunction, and response provide an overview of the versatility enabled by these cultural systems.

人体体外血液屏障模型:结构与应用。
血液屏障是运输重要分子的关键点,也是抵御毒素的防线。对这些屏障进行体外建模是研究其生理学和相关疾病的常用方法。本综述介绍了一种使用适应性强、成本低、半透的悬浮膜来实验模拟人体内三种血液屏障的常用方法:血脑屏障 (BBB)、肠道-血液屏障 (GBB) 和气血屏障 (ABB)。血脑屏障和气血屏障都保护人体不受外界环境的影响,而血脑屏障则保护中枢神经系统不受血液中潜在神经毒剂的影响。这些屏障有几个共同点,包括形成紧密连接、极化细胞单层和循环系统接触。用于模拟屏障解剖结构的细胞结构以及研究功能、功能障碍和反应的应用概述了这些培养系统的多功能性。
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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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