Wastewater surveillance of SARS-CoV-2 corroborates heightened community infection during the initial peak of COVID-19 in Bexar County, Texas.

FEMS microbes Pub Date : 2021-10-13 eCollection Date: 2021-01-01 DOI:10.1093/femsmc/xtab015
Haya Al-Duroobi, Sina V Moghadam, Duc C Phan, Arash Jafarzadeh, Akanksha Matta, Vikram Kapoor
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Abstract

The purpose of this study was to conduct a preliminary assessment of the levels of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater at the Salitrillo Wastewater Treatment Plant in Texas during the initial peak of coronavirus disease 2019 (COVID-19) outbreak. Raw wastewater influent (24 h composite, time-based 1 L samples, n = 13) was collected weekly during June-August 2020. We measured SARS-CoV-2 RNA in wastewater by reverse transcription droplet digital PCR using the same N1 and N2 primer sets as employed in COVID-19 clinical testing. Virus RNA copies for positive samples (77%) ranged from 1.4 × 102 to 4.1 × 104 copies per liter of wastewater, and exhibited both increasing and decreasing trends, which corresponded well with the COVID-19 weekly infection rate (N1: ρ = 0.558, P = 0.048; N2: ρ = 0.487, P = 0.092). A sharp increase in virus RNA concentrations was observed during July sampling dates, consistent with the highest number of COVID-19 cases reported. This could be attributed to an increase in the spread of COVID-19 infection due to the Fourth of July holiday week gatherings (outdoor gatherings were limited to 100 people during that time). Our data show that wastewater surveillance is an effective tool to determine trends in infectious disease prevalence, and provide complementary information to clinical testing.

Abstract Image

Abstract Image

对 SARS-CoV-2 的废水监测证实,在得克萨斯州贝萨县 COVID-19 最初的高峰期,社区感染率有所上升。
本研究旨在对 2019 年冠状病毒病(COVID-19)爆发初期高峰期得克萨斯州 Salitrillo 污水处理厂废水中严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)RNA 的水平进行初步评估。在 2020 年 6 月至 8 月期间,我们每周收集一次原废水进水(24 小时复合、基于时间的 1 L 样品,n = 13)。我们使用与 COVID-19 临床检测中相同的 N1 和 N2 引物组,通过反转录液滴数字 PCR 检测废水中的 SARS-CoV-2 RNA。阳性样本(77%)的病毒 RNA 份数为每升废水 1.4 × 102 至 4.1 × 104 份,呈上升和下降趋势,与 COVID-19 的周感染率十分吻合(N1:ρ = 0.558,P = 0.048;N2:ρ = 0.487,P = 0.092)。在 7 月份的采样日期,病毒 RNA 浓度急剧上升,这与报告的 COVID-19 病例数最多的日期一致。这可能是由于国庆节假期一周的聚会(在此期间,户外聚会人数限制在 100 人以内)导致 COVID-19 感染传播的增加。我们的数据表明,废水监测是确定传染病流行趋势的有效工具,可为临床检测提供补充信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
3.30
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15 weeks
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