What transcriptomics and proteomics can tell us about a high borate perturbed boron tolerant Bacilli strain†

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2023-04-04 DOI:10.1039/D3MO00023K

Subhajit Sen, Sriradha Ganguli and Ranadhir Chakraborty

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Abstract

A variety of genes work together to allow the bacterium Lysinibacillus sp. OL1 to survive and grow under B-stress circumstances. This bacterium was previously identified and described from agricultural soil treated with a boron fertilizer. The effects of B-stress on OL1 cells cultured in the presence of 200 mM boric acid were evaluated as changes in the log-phase cell transcriptome and proteome. OL1 has been found to upregulate all genes involved in producing critical macromolecules when exposed to B-stress. It was also observed that genes governing energy supply lines were in higher expression stages, indicating that they were more likely to support the increased production of macromolecules and stress-induced proteins, such as efflux proteins, to reduce boron damage and prevent boron accumulation inside the cell. It has been explained how the hub genes and bottleneck genes cooperate to survive boron stress and support bacterial growth. The proteome results have significantly confirmed the boron tolerance paradigm. Thus, the current study has improved our understanding of the bacterial B-stress response mechanism and opened new research directions.

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转录组学和蛋白质组学可以告诉我们一个高硼酸盐干扰的耐硼芽孢杆菌菌株†

多种基因共同作用，使细菌Lysinibacillus sp. OL1在b逆境环境下存活和生长。这种细菌以前是在硼肥处理过的农业土壤中发现和描述的。b -应激对200 mM硼酸环境下培养的OL1细胞的影响通过对数相细胞转录组和蛋白质组的变化来评估。当暴露于b应激时，OL1已被发现上调所有参与产生关键大分子的基因。还观察到，控制能量供应线的基因处于较高的表达阶段，表明它们更有可能支持大分子和应激诱导蛋白(如外排蛋白)的增加生产，以减少硼损害并防止硼在细胞内积聚。已经解释了枢纽基因和瓶颈基因如何协同生存硼胁迫和支持细菌生长。蛋白质组学结果显著地证实了硼耐受性范式。因此，本研究提高了我们对细菌b应激反应机制的认识，开辟了新的研究方向。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.