The mechanism of chronic unpredictable mild stress induced high blood pressure in rats: a proteomic and targeted metabolomic analysis†

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular omics Pub Date : 2023-03-07 DOI:10.1039/D2MO00332E

Hongxia Zhao, Qiong Wu, Na Li and Yongchun Chen

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Abstract

Chronic stress, a leading factor for high blood pressure (BP) and even hypertension, affects health quality seriously. However, the management is rather difficult in our rapidly developing modern society, and the underlying mechanism that caused hypertension remains incompletely understood. In this study, we established a rat model of high BP induced by chronic unpredictable mild stress (CUMS). The results showed that CUMS increased the BP and heart rate, as well as the concentrations of CORT, NA, and ACTH. Based on tandem mass tag (TMT)-labeled proteomics, 13 proteins changed in RVLM. Then, targeted metabolomics together with real-time qPCR were applied to validate the levels of the biomolecules quantitatively. The related molecules were confirmed to reveal that CUMS has a great role in the upregulation of muscle contraction, synthesis of cAMP and transport of metals, while down-regulating ralaxin signaling. This finding facilitates a better understanding of the mechanism of hypertension induced by chronic stress and could provide an insight into the prevention and treatment of hypertension.

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慢性不可预测的轻度应激诱导大鼠高血压的机制:蛋白质组学和靶向代谢组学分析

慢性应激是导致高血压甚至高血压的主要因素，严重影响健康质量。然而，在快速发展的现代社会中，高血压的管理相当困难，其发病机制也不完全清楚。在本研究中，我们建立了慢性不可预测轻度应激(CUMS)引起的大鼠高血压模型。结果显示，CUMS使血压和心率升高，CORT、NA和ACTH浓度升高。基于串联质量标签(TMT)标记的蛋白质组学，RVLM中有13个蛋白发生了变化。然后，应用靶向代谢组学和实时qPCR技术定量验证生物分子的水平。通过对相关分子的证实，揭示了CUMS在上调肌肉收缩、cAMP合成和金属转运，下调松弛素信号传导等方面具有重要作用。这一发现有助于更好地理解慢性应激引起高血压的机制，并为高血压的预防和治疗提供新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

5.40

自引率

3.40%

发文量

期刊介绍： Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.