Influence of feedstock source on the development of polyhydroxyalkanoates-producing mixed microbial cultures in continuously stirred tank reactors

IF 4.5 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

New biotechnology Pub Date : 2023-09-25 DOI:10.1016/j.nbt.2023.05.005

Elisa Clagnan, Fabrizio Adani

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引用次数: 0

Abstract

Polyhydroxyalkanoates (PHAs) are the new frontier of bioplastic production; however, research is needed to develop and characterise efficient mixed microbial communities (MMCs) for their application with a multi-feedstock approach. Here, the performance and composition of six MMCs developed from the same inoculum on different feedstocks were investigated through Illumina sequencing to understand community development and identify possible redundancies in terms of genera and PHA metabolism. High PHA production efficiencies (>80% mg COD_PHA mg^-1 COD_OA-consumed) were seen across all samples, but differences in the organic acids (OAs) composition led to different ratios of the monomers poly(3-hydroxybutyrate) (3HB) to poly(3-hydroxyvalerate) (3HV). Communities differed across all feedstocks, with enrichments in specific PHA-producing genera, but analysis of potential enzymatic activity identified a certain degree of functional redundancy, possibly leading to the general high efficiency seen in PHA production from all feedstocks. Leading PHAs producers across all feedstocks were identified in genera such as Thauera, Leadbetterella, Neomegalonema and Amaricoccus.

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原料来源对在连续搅拌釜式反应器中生产混合微生物培养物的聚羟基烷酸酯发展的影响

聚羟基烷酸酯（PHA）是生物塑料生产的新前沿；然而，需要研究开发和表征有效的混合微生物群落（MMCs），以便通过多原料方法进行应用。在这里，通过Illumina测序研究了在不同原料上由相同接种物开发的六种MMC的性能和组成，以了解群落发育，并确定属和PHA代谢方面可能的冗余。在所有样品中都观察到高的PHA生产效率（>；消耗80%mg CODPHA mg-1 CODOA），但有机酸（OA）组成的差异导致单体聚（3-羟基丁酸酯）（3HB）与聚（3-羟戊酸酯）（3HV）的比例不同。所有原料的群落不同，在特定的PHA生产属中富集，但对潜在酶活性的分析发现了一定程度的功能冗余，可能导致所有原料的PHA生产普遍高效。在Thauera、Leadbetterella、Neomegalonema和Amaricoccus等属中确定了所有原料中领先的PHA生产商。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New biotechnology 生物-生化研究方法

CiteScore

11.40

自引率

1.90%

发文量

审稿时长

1 months

期刊介绍： New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.