Variation of growth and transcriptome responses to arbuscular mycorrhizal symbiosis in different foxtail millet lines.

IF 3.4 3区 生物学 Q1 Agricultural and Biological Sciences
Ou-Chi Chang, Wei-Yi Lin
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引用次数: 0

Abstract

Background: Arbuscular mycorrhizal fungi (AMF) have been applied to promote the growth of different crop species, but knowledge about the impacts of symbiosis on foxtail millet at the physiological and molecular levels have remained limited. In this study, we compared the mycorrhization phenotypes of one cultivar and three different landraces and performed a comprehensive transcriptomic analysis to assess the effects of genetic variation on the responses to symbiosis.

Results: Our results showed that colonization by AMF did not enhance biomass accumulation but significantly increased grain production only in three lines. More than 2,000 genes were affected by AMF colonization in all lines. Most AM symbiosis-conserved genes were induced, but the induction levels varied between lines. Gene Ontology (GO) analysis showed that Biological Function terms related to nitrogen transport and assimilation were only enriched in TT8. Similarly, two of phosphate starvation-induced phosphate transporters were only simultaneously downregulated in TT8. In the other two lines, the enrichment of GO terms associated with cell wall reorganization and lignification was observed, though the effects were different.

Conclusion: This study reveals the impacts of genetic variation of millet lines on the responses to AM symbiosis and provides information regarding AMF application for millet production.

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不同谷子品系生长和转录组对丛枝菌根共生的响应变化。
背景:丛枝菌根真菌(AMF)已被应用于促进不同作物的生长,但对其在生理和分子水平上对谷子的影响的认识仍然有限。在这项研究中,我们比较了一个栽培品种和三个不同地方品种的菌根表型,并进行了全面的转录组学分析,以评估遗传变异对共生反应的影响。结果:在3个品系中,AMF的定殖并没有增加生物量积累,但显著提高了籽粒产量。所有品系中有2000多个基因受AMF定殖影响。大部分AM共生保守基因均被诱导,但诱导水平在不同系间存在差异。基因本体(Gene Ontology, GO)分析表明,与氮转运和同化相关的生物学功能术语仅在TT8中富集。同样,两种磷酸盐饥饿诱导的磷酸盐转运蛋白在TT8中只同时下调。在另外两个品系中,观察到与细胞壁重组和木质化相关的氧化石墨烯术语的富集,尽管效果不同。结论:本研究揭示了谷子品系遗传变异对AM共生响应的影响,为AMF在谷子生产中的应用提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Botanical Studies
Botanical Studies 生物-植物科学
CiteScore
5.50
自引率
2.90%
发文量
32
审稿时长
2.4 months
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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