Novel pan-lineage VP1 specific degenerate primers for precise genetic characterization of serotype O foot and mouth disease virus circulating in India.

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES
Sagar Ashok Khulape, Jitendra Kumar Biswal, Chandrakanta Jana, Saravanan Subramaniam, Rabindra Prasad Singh
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引用次数: 0

Abstract

Analysis of the VP1 gene sequence of the foot and mouth disease virus (FMDV) is critical to understanding viral evolution and disease epidemiology. A standard set of primers have been used for the detection and sequence analysis of the VP1 gene of FMDV directly from suspected clinical samples with limited success. The study validated VP1-specific degenerate primer-based reverse transcription polymerase chain reaction (RT-PCR) for the qualitative detection and sequencing of serotype O FMDV lineages circulating in India. The novel degenerate primer-based RT-PCR amplifying the VP1 gene can circumvent the genetic heterogeneity observed in viruses after cell culture adaptation and facilitate precise viral gene sequence analysis from clinical samples.

Abstract Image

Abstract Image

新型泛谱系VP1特异性退化引物用于印度流行的血清O型口蹄疫病毒的精确遗传表征
分析口蹄疫病毒(FMDV) VP1基因序列对了解病毒进化和疾病流行病学具有重要意义。一套标准引物已被用于直接从疑似临床样本中检测和序列分析FMDV的VP1基因,但成功率有限。该研究验证了基于vp1特异性退化引物的逆转录聚合酶链反应(RT-PCR)在印度流行的血清O型FMDV谱系的定性检测和测序。新型的基于退化引物的RT-PCR扩增VP1基因,可以规避病毒细胞培养适应后的遗传异质性,便于从临床样本中精确分析病毒基因序列。
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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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