LncRNA SSTR5-AS1 as a Prognostic Marker Promotes Cell Proliferation and Epithelial-to-Mesenchymal Transition in Prostate Cancer.

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/CritRevEukaryotGeneExpr.2022042183

Shuai Yuan, Jianlong Bi, Yangang Zhang

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引用次数: 1

Abstract

This study is aimed to investigate the clinical significance and biological function of long non-coding RNA somatostatin receptor 5 antisense RNA 1 (SSTR5-AS1) in prostate cancer (PCa). Here, we found that SSTR5-AS1 expression was upregulated in PCa tissues compared with adjacent tissues using quantitative real time PCR analysis. The results from Chi-square test showed that increased SSTR5-AS1 expression levels were correlated with preoperative prostate specific antigen, tumor stage and lymph node metastasis. Kaplan-Meier survival curve described patients with high SSTR5-AS1 expression level showed poor survival. Univariate and multivariate cox regression analysis further identified SSTR5-AS1 expression as a poor independent prognostic factor for PCa patients. Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine incorporation assay, wound-healing assay and Transwell assay were performed to investigate the functional role of SSTR5-AS1 in PCa cells. The in vitro results indicated that SSTR5-AS1 knockdown inhibited, while SSTR5-AS1 overexpression promoted the proliferation, migration, and invasion of PCa cells. At molecular level, SSTR5-AS1 knockdown downregulated the protein levels of proliferating cell nuclear antigen, N-cadherin and vimentin, and upregulated E-cadherin expression in PC-3 cells. SSTR5-AS1 overexpression obtained opposite results on these protein markers in DU145 cells. In conclusion, these findings indicated that SSTR5-AS1 promotes PCa cell behaviors, which might provide a potential therapeutic target for PCa patients.

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LncRNA SSTR5-AS1作为预后标志物促进前列腺癌细胞增殖和上皮-间质转化

本研究旨在探讨长链非编码RNA生长抑素受体5反义RNA 1 (SSTR5-AS1)在前列腺癌(PCa)中的临床意义和生物学功能。本研究通过实时定量PCR分析发现，与癌旁组织相比，SSTR5-AS1在癌旁组织中的表达上调。卡方检验结果显示，SSTR5-AS1表达水平升高与术前前列腺特异性抗原、肿瘤分期及淋巴结转移相关。Kaplan-Meier生存曲线描述了SSTR5-AS1高表达水平的患者生存率较差。单因素和多因素cox回归分析进一步发现，SSTR5-AS1表达是PCa患者的一个较差的独立预后因素。采用细胞计数试剂盒-8 (CCK-8)法、5-乙基-2′-脱氧尿苷掺入法、伤口愈合法和Transwell法研究SSTR5-AS1在PCa细胞中的功能作用。体外实验结果表明，SSTR5-AS1敲低抑制了PCa细胞的增殖、迁移和侵袭，而SSTR5-AS1过表达促进了PCa细胞的增殖、迁移和侵袭。在分子水平上，SSTR5-AS1敲低可下调PC-3细胞中增殖细胞核抗原、N-cadherin和vimentin蛋白水平，上调E-cadherin表达。在DU145细胞中，SSTR5-AS1过表达获得了相反的结果。综上所述，这些发现表明SSTR5-AS1可以促进PCa细胞的行为，可能为PCa患者提供潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.