First molecular characterization of Escherichia coli O157:H7 isolates from clinical samples in Paraguay using whole-genome sequencing

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-04-01 DOI:10.1016/j.ram.2022.11.002

Natalie Weiler , Lucia Jazmín Martínez , Josefina Campos , Tomas Poklepovich , Maria Veronica Orrego , Flavia Ortiz , Mercedes Alvarez , Karina Putzolu , Gisela Zolezzi , Elisabeth Miliwebsky , Isabel Chinen

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引用次数: 1

Abstract

Escherichia coli O157:H7 is a foodborne pathogen implicated in numerous outbreaks worldwide that has the ability to cause extra-intestinal complications in humans. The Enteropathogens Division of the Central Public Health Laboratory (CPHL) in Paraguay is working to improve the genomic characterization of Shiga toxin-producing E. coli (STEC) to enhance laboratory-based surveillance and investigation of foodborne disease outbreaks. Whole genome sequencing (WGS) is proposed worldwide to be used in the routine laboratory as a high-resolution tool that allows to have all the results in a single workflow. This study aimed to carry out for the first time, the genomic characterization by WGS of nine STEC O157:H7 strains isolated from human samples in Paraguay. We were able to identify virulence and resistance mechanisms, MLST subtype, and even establish the phylogenetic relationships between isolates. Furthermore, we detected the presence of strains belonging to hypervirulent clade 8 in most of the isolates studied.

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利用全基因组测序首次对巴拉圭临床样本中分离的大肠杆菌O157:H7进行分子鉴定

大肠杆菌O157:H7是一种食源性病原体，与世界各地的许多疫情有关，能够引起人类肠道外并发症。巴拉圭中央公共卫生实验室(CPHL)的肠病原体司正在努力改进产志贺毒素大肠杆菌(STEC)的基因组特征，以加强对食源性疾病暴发的实验室监测和调查。全基因组测序(WGS)作为一种高分辨率的工具，在世界范围内被建议用于常规实验室，它允许在一个工作流程中获得所有结果。本研究旨在首次利用WGS对从巴拉圭人标本中分离的9株产志贺毒素大肠杆菌O157:H7菌株进行基因组鉴定。我们能够确定毒力和耐药机制，MLST亚型，甚至建立分离株之间的系统发育关系。此外，我们在大多数研究的分离株中检测到属于高毒力分支8的菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.