Comparison of HPV 16/18 Genotyping and p16/Ki67 Dual Staining for Detection of High-Grade Cervical Lesion in Patients with Low-Grade Cervical Smears.

IF 0.7 Q4 OBSTETRICS & GYNECOLOGY

Journal of Obstetrics and Gynecology of India Pub Date : 2023-06-01 Epub Date: 2023-01-09 DOI:10.1007/s13224-022-01731-x

Saloni Chadha, Gauri Gandhi, Suresh T Hedau, Ruchika Gupta

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引用次数: 0

Abstract

Objective: To triage low-grade cervical smears (ASCUS/LSIL) by HPV 16/18 genotyping and dual staining with p16/Ki67 and to compare the sensitivity and specificity of these two triage methods for detection of high-grade cervical intraepithelial neoplasia (HGCIN).

Methods: In this prospective cross-sectional study, we evaluated a total of 89 women with low-grade smears (54 ASCUS, 35 LSIL) recruited from a tertiary care hospital. All patients underwent colposcopy guided cervical biopsy. Histopathology was used as gold standard. All samples were subjected to HPV 16/18 genotyping (excluding 9) using DNA PCR and p16/Ki67 dual staining (excluding 4) using Roche® kit. We then compared the two triage methods to detect high-grade cervical lesions.

Results: Overall, in all low-grade smears sensitivity, specificity and accuracy of HPV 16/18 genotyping, was found to be 66.7%, 77.1% and 76.2% respectively (p = 0.03). In low-grade smears sensitivity, specificity and accuracy of dual staining, was found to be 66.7%, 84.8% and 83.5% respectively (p = 0.01).

Conclusions: Overall, in all low-grade smears the sensitivity of the two tests was comparable. However, dual staining had a higher specificity and accuracy than HPV 16/18 genotyping. It was concluded that both are effective triage methods but dual staining had a better performance than HPV 16/18 genotyping.

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HPV16/18基因分型和p16/Ki67双染色检测低级别宫颈涂片患者高级别宫颈病变的比较。

目的：通过HPV16/18基因分型和p16/Ki67双染色对低级别宫颈涂片（ASCUS/LSIL）进行分型，并比较这两种分型方法检测高级别宫颈上皮内瘤变（HGCIN）的敏感性和特异性，我们评估了从一家三级护理医院招募的89名低级别涂片女性（54名ASCUS，35名LSIL）。所有患者均接受阴道镜引导下的宫颈活检。组织病理学被用作金标准。所有样本均使用DNA PCR进行HPV 16/18基因分型（不包括9个），并使用Roche®试剂盒进行p16/Ki67双重染色（不包括4个）。然后，我们比较了两种分诊方法来检测高级别宫颈病变。结果：总的来说，在所有低级别涂片中，HPV 16/18基因分型的敏感性、特异性和准确性分别为66.7%、77.1%和76.2%（p = 在低级别涂片中，双重染色的敏感性、特异性和准确性分别为66.7%、84.8%和83.5%（p = 0.01）。结论：总的来说，在所有低级别的涂片中，两种测试的敏感性是可比较的。然而，双重染色比HPV 16/18基因分型具有更高的特异性和准确性。结果表明，这两种方法都是有效的分型方法，但双重染色比HPV 16/18基因分型具有更好的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Obstetrics and Gynecology of India OBSTETRICS & GYNECOLOGY-

CiteScore

1.30

自引率

0.00%

发文量

124

期刊介绍： Journal of Obstetrics and Gynecology of India (JOGI) is the official journal of the Federation of Obstetrics and Gynecology Societies of India (FOGSI). This is a peer- reviewed journal and features articles pertaining to the field of obstetrics and gynecology. The Journal is published six times a year on a bimonthly basis. Articles contributed by clinicians involved in patient care and research, and basic science researchers are considered. It publishes clinical and basic research of all aspects of obstetrics and gynecology, community obstetrics and family welfare and subspecialty subjects including gynecological endoscopy, infertility, oncology and ultrasonography, provided they have scientific merit and represent an important advance in knowledge. The journal believes in diversity and welcomes and encourages relevant contributions from world over. The types of articles published are: · Original Article· Case Report · Instrumentation and Techniques · Short Commentary · Correspondence (Letter to the Editor) · Pictorial Essay