The Nasal Innate Immune Proteome After Saline Irrigation: A Pilot Study in Healthy Individuals.

IF 2.5 3区医学 Q1 OTORHINOLARYNGOLOGY

American Journal of Rhinology & Allergy Pub Date : 2023-07-01 DOI:10.1177/19458924231159176

Emily A Harcourt-Smith, Emerson T Krstic, Belinda J Soekov-Pearce, Alex D Colella, Nusha Chegeni, Timothy K Chataway, Charmaine M Woods, Kamelya Aliakbari, A Simon Carney

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引用次数: 1

Abstract

Background: Previous research has shown diminished nasal immune function following nasal saline irrigation (NSI), returning to baseline at 6 hours. The aim of this study was to examine the immune nasal proteome before and after 14 days of nasal irrigation.

Methods: Seventeen healthy volunteers received either isotonic (IsoSal) or low salt (LowNa) NSI. Nasal secretions were collected before and 30 min after NSI at baseline and again after 14 days. Specimens were analyzed using mass spectrometry to detect proteins of relevance to nasal immune function.

Results: One thousand eight hundred and sixty-five proteins were identified with significant changes in 71 proteins, of which 23 were identified as part of the innate immune system. Baseline analysis demonstrated an increase of 9 innate proteins after NSI, most after IsoSal. After 14 days, a greater increase in innate peptides was present, with most now in the LowNa group. When NSI solutions were compared, a significant increase in 4 innate proteins, including a 211% in lysozyme, was detected in the LowNa group.

Conclusion: LowNa NSI demonstrates evidence of improving the innate immune secretions, especially lysozyme, in healthy volunteers.

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生理盐水冲洗后鼻腔先天免疫蛋白质组:健康个体的初步研究

背景:先前的研究表明，鼻盐水冲洗(NSI)后，鼻腔免疫功能下降，在6小时后恢复到基线。本研究的目的是检测鼻腔冲洗前后14天的免疫鼻腔蛋白质组。方法:17名健康志愿者接受等渗(IsoSal)或低盐(LowNa)自伤。在基线NSI前和30分钟收集鼻分泌物，14天后再次收集鼻分泌物。采用质谱法对标本进行分析，检测与鼻腔免疫功能相关的蛋白质。结果:共鉴定出1665个蛋白，其中71个蛋白发生了显著变化，其中23个蛋白被鉴定为先天免疫系统的一部分。基线分析显示，NSI后9种先天蛋白增加，大多数在issal后增加。14天后，先天多肽出现了更大的增加，现在大多数出现在LowNa组。当比较NSI溶液时，在LowNa组检测到4种天然蛋白显著增加，其中溶菌酶增加了211%。结论:LowNa NSI可改善健康志愿者的先天免疫分泌，尤其是溶菌酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Rhinology & Allergy 医学-耳鼻喉科学

CiteScore

5.60

自引率

11.50%

发文量

审稿时长

4-8 weeks

期刊介绍： The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.