Great diversity of KSα sequences from bat-associated microbiota suggests novel sources of uncharacterized natural products.

FEMS microbes Pub Date : 2022-04-18 eCollection Date: 2022-01-01 DOI:10.1093/femsmc/xtac012
Paris S Salazar-Hamm, Jennifer J Marshall Hathaway, Ara S Winter, Nicole A Caimi, Debbie C Buecher, Ernest W Valdez, Diana E Northup
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引用次数: 1

Abstract

Polyketide synthases (PKSs) are multidomain enzymes in microorganisms that synthesize complex, bioactive molecules. PKS II systems are iterative, containing only a single representative of each domain: ketosynthase alpha (KS[Formula: see text]), ketosynthase beta and the acyl carrier protein. Any gene encoding for one of these domains is representative of an entire PKS II biosynthetic gene cluster (BGC). Bat skin surfaces represent an extreme environment prolific in Actinobacteria that may constitute a source for bioactive molecule discovery. KS[Formula: see text] sequences were obtained from culturable bacteria from bats in the southwestern United States. From 467 bat bacterial isolates, we detected 215 (46%) had KS[Formula: see text] sequences. Sequencing yielded 210 operational taxonomic units, and phylogenetic placement found 45 (21%) shared <85% homology to characterized metabolites. Additionally, 16 Actinobacteria genomes from the bat microbiome were analyzed for biosynthetic capacity. A range of 69-93% of the BGCs were novel suggesting the bat microbiome may contain valuable uncharacterized natural products. Documenting and characterizing these are important in understanding the susceptibility of bats to emerging infectious diseases, such as white-nose syndrome. Also noteworthy was the relationship between KS [Formula: see text] homology and total BGC novelty within each fully sequenced strain. We propose amplification and detection of KS[Formula: see text] could predict a strain's global biosynthetic capacity.

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来自蝙蝠相关微生物群的ksa α序列的巨大多样性表明了未表征的天然产物的新来源。
聚酮合成酶(pks)是微生物中合成复杂生物活性分子的多结构域酶。PKS II系统是迭代的,每个结构域只包含一个代表:酮合酶α (KS[公式:见文本]),酮合酶β和酰基载体蛋白。任何编码这些结构域的基因都是整个PKS II生物合成基因簇(BGC)的代表。蝙蝠皮肤表面代表了放线菌丰富的极端环境,可能构成生物活性分子发现的来源。KS[公式:见文本]序列是从美国西南部蝙蝠的可培养细菌中获得的。从467株蝙蝠细菌分离株中,我们检测到215株(46%)具有KS序列。测序获得210个操作分类单位,系统发育定位发现45个(21%)共享
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CiteScore
3.30
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