Association of carbapenem and multidrug resistance with the expression of efflux pump-encoding genes in Pseudomonas aeruginosa clinical isolates.

IF 1.3 4区医学 Q4 IMMUNOLOGY

Acta microbiologica et immunologica Hungarica Pub Date : 2023-06-16 DOI:10.1556/030.2023.02029

Shaghayegh Yousefi, Maryam Nazari, Rashid Ramazanzadeh, Amirhossein Sahebkar, Elham Safarzadeh, Farzad Khademi

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引用次数: 0

Abstract

Efflux pumps play an important role in the emergence of antibiotic-resistant Pseudomonas aeruginosa strains. The present study aimed to assess the expression of the MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM efflux pumps in carbapenem-resistant and multidrug-resistant (MDR) P. aeruginosa strains isolated from clinical specimens between June 2019 and January 2022 in Ardabil city. The presence of efflux pump-encoding genes, i.e. mexA, mexC, mexE, and mexY, was assessed using the polymerase chain reaction (PCR) technique in 48 carbapenem-resistant and MDR P. aeruginosa strains. Real-time reverse transcription PCR was employed to evaluate the expression levels of mexA, mexC, mexE, and mexY genes. All 48 carbapenem-resistant and MDR P. aeruginosa strains harbored efflux pump-encoding genes including mexA, mexC, mexE, and mexY according to the PCR results. Overexpression of the MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM efflux pumps was detected in 75% (n = 36), 83.3% (n = 40), 10.4% (n = 5) and 41.6% (n = 20) of the clinical isolates of P. aeruginosa, respectively. This study revealed that the presence and overexpression of efflux pumps are associated with the emergence of carbapenem-resistant and MDR P. aeruginosa strains. Therefore, research on efflux pump inhibitors of P. aeruginosa will be a worthwhile endeavor to increase the clinical efficiency of available antibiotics and prevent ensuing treatment failure.

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铜绿假单胞菌临床分离株碳青霉烯类和多药耐药与外排泵编码基因表达的关系

外排泵在耐药铜绿假单胞菌菌株的出现中起重要作用。本研究旨在评估2019年6月至2022年1月阿达比勒市临床标本中分离的碳青霉烯耐药和多药耐药(MDR)铜绿假单胞菌中MexAB-OprM、mexd - oprj、MexEF-OprN和mexx - oprm外排泵的表达情况。采用聚合酶链反应(PCR)技术对48株碳青霉烯耐药和耐多药铜绿假单胞菌进行了外排泵编码基因mexA、mexC、mexE和mexY的检测。实时反转录PCR检测mexA、mexC、mexE、mexY基因的表达水平。PCR结果显示，48株耐碳青霉烯和耐多药铜绿假单胞菌均含有外排泵编码基因，包括mexA、mexC、mexE和mexY。在铜绿假单胞菌的临床分离株中，分别有75% (n = 36)、83.3% (n = 40)、10.4% (n = 5)和41.6% (n = 20)检测到mexaf - oprm、MexCD-OprJ、MexEF-OprN和mexx - oprm外排泵过表达。本研究表明，外排泵的存在和过表达与碳青霉烯耐药和耐多药铜绿假单胞菌菌株的出现有关。因此，研究铜绿假单胞菌的外排泵抑制剂将是一项值得努力的工作，以提高现有抗生素的临床效率，防止随后的治疗失败。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta microbiologica et immunologica Hungarica IMMUNOLOGY-MICROBIOLOGY

CiteScore

2.30

自引率

13.30%

发文量

审稿时长

>12 weeks

期刊介绍： AMIH is devoted to the publication of research in all fields of medical microbiology (bacteriology, virology, parasitology, mycology); immunology of infectious diseases and study of the microbiome related to human diseases.