Alginate/chitosan hydrogels as perspective transport systems for cefotaxime

IF 3.2 4区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biopolymers Pub Date : 2023-06-15 DOI:10.1002/bip.23555

Svetlana V. Shilova, Grigory M. Mirgaleev, Ksenia A. Romanova, Yury G. Galyametdinov

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引用次数: 0

Abstract

This work reports synthesis of pH-responsive alginate/chitosan hydrogel spheres with the average diameter of 2.0 ± 0.05 mm, which contain cefotaxime that is an antibiotic of the cefalosporine group. The spheres provided the cefotaxime encapsulation efficiency of 95 ± 1%. An in vitro release of cefotaxime from the spheres in the media that simulate human biological fluids in peroral delivery conditions was found to be a pH-dependent process. The analysis of cefotaxime release kinetics by the Korsmeyer–Peppas model revealed a non-Fickian mechanism of its diffusion, which may be related to intermolecular interactions occurring between the antibiotic and chitosan. Conductometry, UV spectroscopy, and IR spectroscopy were used to study complexation of chitosan with cefotaxime in aqueous media with varied pH, characterize the composition of the complexes, and calculate their stability constants. The composition of the cefotaxime–chitosan complexes was found to correspond to the 1.0:4.0 and 1.0:2.0 molar ratios of the components at pH 2.0 and 5.6, respectively. Quantum chemical modeling was used to evaluate energy characteristics of chitosan–cefotaxime complexation considering the influence of a solvent.

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海藻酸盐/壳聚糖水凝胶作为头孢噻肟的前景转运系统。

本工作报道了平均直径为2.0的pH响应性藻酸盐/壳聚糖水凝胶球体的合成 ± 0.05 mm，其含有头孢噻肟，头孢噻肟是头孢菌素组的抗生素。这些球体提供了95的头孢噻肟包封效率 ± 1%。头孢噻肟在模拟人体生物流体的介质中从球体中在经口给药条件下的体外释放被发现是一个pH依赖性过程。Korsmeyer-Peppas模型对头孢噻肟释放动力学的分析揭示了其扩散的非菲克机制，这可能与抗生素和壳聚糖之间发生的分子间相互作用有关。采用电导法、紫外光谱法和红外光谱法研究了壳聚糖与头孢噻肟在不同pH水介质中的络合反应，表征了配合物的组成，并计算了其稳定常数。发现头孢噻肟-壳聚糖复合物的组成对应于pH下组分的1.0:4.0和1.0:2.0摩尔比分别为2.0和5.6。考虑溶剂的影响，采用量子化学模型评价了壳聚糖-头孢噻肟络合反应的能量特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biopolymers 生物-生化与分子生物学

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Founded in 1963, Biopolymers publishes strictly peer-reviewed papers examining naturally occurring and synthetic biological macromolecules. By including experimental and theoretical studies on the fundamental behaviour as well as applications of biopolymers, the journal serves the interdisciplinary biochemical, biophysical, biomaterials and biomedical research communities.