Analysis of fatty acid composition and sensitivity to dietary n-3 PUFA intervention of mouse n-3 PUFA-enriched tissues/organs

IF 3
Hui Zhang , Yannan He , Chunyan Song , Zhenglong Chai , Chundi Liu , Shuben Sun , Qiuhan Huang , Canxia He , Xiaohong Zhang , Yuping Zhou , Feng Zhao
{"title":"Analysis of fatty acid composition and sensitivity to dietary n-3 PUFA intervention of mouse n-3 PUFA-enriched tissues/organs","authors":"Hui Zhang ,&nbsp;Yannan He ,&nbsp;Chunyan Song ,&nbsp;Zhenglong Chai ,&nbsp;Chundi Liu ,&nbsp;Shuben Sun ,&nbsp;Qiuhan Huang ,&nbsp;Canxia He ,&nbsp;Xiaohong Zhang ,&nbsp;Yuping Zhou ,&nbsp;Feng Zhao","doi":"10.1016/j.plefa.2023.102568","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>n-3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA; C22:6 n3) and eicosapentaenoic acid (EPA; C20:5 n3), are of concern for their health-promoting effects such as anti-inflammatory, but the tissue selectivity for n-3 PUFA (i.e., which tissues and organs are rich in n-3 PUFA) is still not well known. In addition, it is unclear which tissues and organs are more sensitive to n-3 PUFA intervention. These unresolved issues have greatly hindered the exploring of the health benefits of n-3 PUFA.</p></div><div><h3>Methods</h3><p>Twenty-four 7-week-old male C57BL/6 J mice were assigned to the control, fish oil, DHA, and EPA groups. The last three groups were given a 4-week oral intervention of fatty acids in ethyl ester (400 mg/kg bw). The fatty acid profiles in 27 compartments were determined by gas chromatography.</p></div><div><h3>Results</h3><p>The proportion of long-chain n-3 PUFA (the total relative percentage of EPA, DPA n3, and DHA) was analyzed. Eight tissues and organs, including the brain (cerebral cortex, hippocampus, hypothalamus) and peripheral organs (tongue, quadriceps, gastrocnemius, kidney, and heart) were determined as being n-3 PUFA-enriched tissues and organs, owing to their high n-3 PUFA levels. The highest n-3 PUFA content was observed in the tongue for the first time. Notably, the content of linoleic acid (LA; C18:2 n6c) in peripheral organs was observed to be relatively high compared with that in the brain. Interestingly, the proportions of EPA in the kidney, heart, quadriceps, gastrocnemius, and tongue increased more markedly after the EPA intervention than after the DHA or fish oil intervention. As expected, the levels of proinflammatory arachidonic acid (AA; C20:4 n6) in the kidney, quadriceps, and tongue were markedly decreased after the three dietary interventions.</p></div><div><h3>Conclusion</h3><p>Peripheral tissues and organs, including the tongue, quadriceps, gastrocnemius, kidney, and heart, besides the brain, showed obvious tissue selectivity for n-3 PUFA. In the whole body of mice, the tongue exhibits the strongest preference for n-3 PUFA, with the highest proportion of n-3 PUFA. Moreover, these peripheral tissues and organs, especially the kidney, are more sensitive to dietary EPA administration in comparison with the brain.</p></div>","PeriodicalId":94179,"journal":{"name":"Prostaglandins, leukotrienes, and essential fatty acids","volume":"192 ","pages":"Article 102568"},"PeriodicalIF":3.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and essential fatty acids","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0952327823000376","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose

n-3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA; C22:6 n3) and eicosapentaenoic acid (EPA; C20:5 n3), are of concern for their health-promoting effects such as anti-inflammatory, but the tissue selectivity for n-3 PUFA (i.e., which tissues and organs are rich in n-3 PUFA) is still not well known. In addition, it is unclear which tissues and organs are more sensitive to n-3 PUFA intervention. These unresolved issues have greatly hindered the exploring of the health benefits of n-3 PUFA.

Methods

Twenty-four 7-week-old male C57BL/6 J mice were assigned to the control, fish oil, DHA, and EPA groups. The last three groups were given a 4-week oral intervention of fatty acids in ethyl ester (400 mg/kg bw). The fatty acid profiles in 27 compartments were determined by gas chromatography.

Results

The proportion of long-chain n-3 PUFA (the total relative percentage of EPA, DPA n3, and DHA) was analyzed. Eight tissues and organs, including the brain (cerebral cortex, hippocampus, hypothalamus) and peripheral organs (tongue, quadriceps, gastrocnemius, kidney, and heart) were determined as being n-3 PUFA-enriched tissues and organs, owing to their high n-3 PUFA levels. The highest n-3 PUFA content was observed in the tongue for the first time. Notably, the content of linoleic acid (LA; C18:2 n6c) in peripheral organs was observed to be relatively high compared with that in the brain. Interestingly, the proportions of EPA in the kidney, heart, quadriceps, gastrocnemius, and tongue increased more markedly after the EPA intervention than after the DHA or fish oil intervention. As expected, the levels of proinflammatory arachidonic acid (AA; C20:4 n6) in the kidney, quadriceps, and tongue were markedly decreased after the three dietary interventions.

Conclusion

Peripheral tissues and organs, including the tongue, quadriceps, gastrocnemius, kidney, and heart, besides the brain, showed obvious tissue selectivity for n-3 PUFA. In the whole body of mice, the tongue exhibits the strongest preference for n-3 PUFA, with the highest proportion of n-3 PUFA. Moreover, these peripheral tissues and organs, especially the kidney, are more sensitive to dietary EPA administration in comparison with the brain.

Abstract Image

富含n-3 PUFA的小鼠组织/器官的脂肪酸组成及对膳食n-3 PUFA干预的敏感性分析
目的-3多不饱和脂肪酸(PUFA),二十二碳六烯酸(DHA;C22:6n3)和二十碳五烯酸(EPA;C20:5n3),因其抗炎等健康促进作用而备受关注,但n-3 PUFA的组织选择性(即哪些组织和器官富含n-3 PUFA)仍不为人所知。此外,尚不清楚哪些组织和器官对n-3 PUFA干预更敏感。这些尚未解决的问题极大地阻碍了n-3 PUFA对健康益处的探索。方法将24只7周大的雄性C57BL/6J小鼠分为对照组、鱼油组、DHA组和EPA组。最后三组接受为期4周的脂肪酸乙酯口服干预(400毫克/公斤体重)。通过气相色谱法测定了27个隔室中的脂肪酸分布。结果分析了长链n-3 PUFA的比例(EPA、DPA-n3和DHA的总相对百分比)。八个组织和器官,包括大脑(大脑皮层、海马体、下丘脑)和外周器官(舌头、股四头肌、腓肠肌、肾脏和心脏),由于其n-3 PUFA水平高,被确定为富含n-3 PUFA的组织和器官。首次在舌头中观察到n-3 PUFA含量最高。值得注意的是,与大脑中相比,外周器官中亚油酸(LA;C18:2 n6c)的含量相对较高。有趣的是,EPA干预后,肾脏、心脏、股四头肌、腓肠肌和舌头中EPA的比例比DHA或鱼油干预后增加得更显著。正如预期的那样,三种饮食干预后,肾脏、股四头肌和舌头中的促炎花生四烯酸(AA;C20:4 n6)水平显著降低。结论除大脑外,舌头、股四头肌、腓肠肌、肾脏和心脏等外周组织和器官对n-3 PUFA表现出明显的组织选择性。在小鼠全身中,舌头对n-3 PUFA表现出最强的偏好,其中n-3 PUFA的比例最高。此外,与大脑相比,这些外周组织和器官,尤其是肾脏,对膳食EPA给药更敏感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
发文量
0
审稿时长
64 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信