Astaxanthin Alleviates Inflammatory Response in Neonatal Necrotizing Enterocolitis Rats by Regulating NOD2/TLR4 Pathway.

IF 2 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Gastroenterology Research and Practice Pub Date : 2023-03-27 eCollection Date: 2023-01-01 DOI:10.1155/2023/6078308

Xuandong Zhang, Yujia Luo, Rui Gu, Zhou Jiang

{"title":"Astaxanthin Alleviates Inflammatory Response in Neonatal Necrotizing Enterocolitis Rats by Regulating NOD2/TLR4 Pathway.","authors":"Xuandong Zhang, Yujia Luo, Rui Gu, Zhou Jiang","doi":"10.1155/2023/6078308","DOIUrl":null,"url":null,"abstract":"Background: Necrotizing enterocolitis (NEC) is often associated with exaggerated activation of inflammatory response. Astaxanthin has been shown in studies to have a positive and advantageous effect on anti-inflammatory response. Hence, it is of great significance to study the protective effect of astaxanthin in NEC disease and its molecular mechanism.Objective: The present study was to investigate whether astaxanthin attenuates NEC rats and to explore its potential mechanism. Material and Methods. Hematoxylin-eosin staining was used to observe the pathological change of the intestinal tissue in NEC rats. Subsequently, we determined the anti-oxidative stress, anti-apoptosis, and anti-inflammation in astaxanthin with enzyme-linked immunosorbent assay kits, TUNEL staining, western blot, and immunohistochemistry assay. Furthermore, we added nucleotide-binding oligomerization domain 2 (NOD2) inhibitor to certify the molecular pathway of the astaxanthin in NEC rats.Results: Astaxanthin improved the pathological changes of the intestinal tissues. It restrained inflammation, oxidative stress, and protected cells from apoptosis in the intestinal tissue and serum of the NEC rats. Moreover, astaxanthin enhanced NOD2, whereas it suppressed toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB) pathway-related proteins. Apart from that, the NOD2 inhibitor offset the protective effect of the astaxanthin towards the NEC rats.Conclusion: The present study indicated that astaxanthin alleviated oxidative stress, inflammatory response, and apoptosis in NEC rats by enhancing NOD2 and inhibiting TLR4 pathway.","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2023 ","pages":"6078308"},"PeriodicalIF":2.0000,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070044/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gastroenterology Research and Practice","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2023/6078308","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Necrotizing enterocolitis (NEC) is often associated with exaggerated activation of inflammatory response. Astaxanthin has been shown in studies to have a positive and advantageous effect on anti-inflammatory response. Hence, it is of great significance to study the protective effect of astaxanthin in NEC disease and its molecular mechanism.

Objective: The present study was to investigate whether astaxanthin attenuates NEC rats and to explore its potential mechanism. Material and Methods. Hematoxylin-eosin staining was used to observe the pathological change of the intestinal tissue in NEC rats. Subsequently, we determined the anti-oxidative stress, anti-apoptosis, and anti-inflammation in astaxanthin with enzyme-linked immunosorbent assay kits, TUNEL staining, western blot, and immunohistochemistry assay. Furthermore, we added nucleotide-binding oligomerization domain 2 (NOD2) inhibitor to certify the molecular pathway of the astaxanthin in NEC rats.

Results: Astaxanthin improved the pathological changes of the intestinal tissues. It restrained inflammation, oxidative stress, and protected cells from apoptosis in the intestinal tissue and serum of the NEC rats. Moreover, astaxanthin enhanced NOD2, whereas it suppressed toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB) pathway-related proteins. Apart from that, the NOD2 inhibitor offset the protective effect of the astaxanthin towards the NEC rats.

Conclusion: The present study indicated that astaxanthin alleviated oxidative stress, inflammatory response, and apoptosis in NEC rats by enhancing NOD2 and inhibiting TLR4 pathway.

Abstract Image

查看原文本刊更多论文

虾青素通过调节 NOD2/TLR4 通路缓解新生儿坏死性小肠结肠炎大鼠的炎症反应

背景：坏死性小肠结肠炎（NEC坏死性小肠结肠炎（NEC）通常与炎症反应的过度激活有关。研究表明，虾青素对抗炎反应具有积极和有利的作用。因此，研究虾青素对NEC疾病的保护作用及其分子机制具有重要意义：本研究旨在探讨虾青素是否能减轻NEC大鼠的病情，并探索其潜在机制。材料和方法。采用血红素-伊红染色法观察NEC大鼠肠道组织的病理变化。随后，我们通过酶联免疫吸附试验、TUNEL染色、Western印迹和免疫组化测定虾青素的抗氧化应激、抗凋亡和抗炎作用。此外，我们还添加了核苷酸结合寡聚化结构域2（NOD2）抑制剂，以证实虾青素在NEC大鼠中的分子途径：结果：虾青素改善了肠道组织的病理变化。结果：虾青素改善了NEC大鼠肠道组织的病理变化，抑制了肠道组织和血清中的炎症、氧化应激和细胞凋亡。此外，虾青素还能增强 NOD2，而抑制收费样受体 4 (TLR4)、核因子-κB (NF-κB)通路相关蛋白。此外，NOD2 抑制剂抵消了虾青素对 NEC 大鼠的保护作用：本研究表明，虾青素通过增强NOD2和抑制TLR4通路，减轻了NEC大鼠的氧化应激、炎症反应和细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.