Decreased Expression of Urethral Caveolin-1, -2, and -3 in the Rat Model of Overactive Bladder: Potential Mediator of Functional Interaction of Urethra and Urinary Bladder.

IF 1.8 3区医学 Q3 UROLOGY & NEPHROLOGY

International Neurourology Journal Pub Date : 2023-03-01 DOI:10.5213/inj.2244236.118

Hyun Jin Cho, Han-Yi Jiao, Sun-Ouck Kim

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Abstract

Purpose: To investigate the effect of detrusor overactivity (DO) on the urethral expression of caveolin (CAV)-1, -2, and -3 of urethra in an animal model of cyclophosphamide (CYP)-induced cystitis rat.

Methods: Female Sprague-Dawley rats were divided into the control group (n=20) and the cystitis group (n=20). Cystitis was induced by intraperitoneal injection of CYP (200 mg/kg). An urodynamic study was done 3 days after the CYP injection to measure functional change of the urinary bladder and urethra. Cellular localization and expression of CAV-1, -2, and -3 in the rat urethra were determined by immunohistochemistry (IHC) and Western blot.

Results: Urodynamic experiments demonstrated a decreased contraction interval in the cystitis group compared to the control (3.9±1.0 minutes vs. 6.6±1.2 minutes, P<0.05). Conversely, contraction pressure increased significantly in the cystitis group compared to the control (22.4±0.7 mmHg vs. 11.5±0.4 mmHg, P<0.05). The urethral pressure was decreased in the cystitis group compared to the control (4.05 ±2.5 mmHg vs. 5.8 ±2.8 mmHg, P <0.05). The IHC and Western blot data showed that CAV-1, -2, and -3 expression decreased significantly in the cystitis group compared control group (P<0.05).

Conclusion: The decreased urethral CAV-1, -2, and -3 in the DO rats suggests that CAVs might be related with the functional change of urethra in association with DO of urinay bladder.

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膀胱过度活动大鼠尿道小窝蛋白-1、-2和-3表达降低:尿道和膀胱功能相互作用的潜在介质

目的:探讨逼尿肌过度活动(DO)对环磷酰胺(CYP)致膀胱炎大鼠尿道小窝蛋白(CAV)-1、-2、-3表达的影响。方法:雌性Sprague-Dawley大鼠分为对照组(n=20)和膀胱炎组(n=20)。腹腔注射CYP (200 mg/kg)诱导膀胱炎。注射CYP 3天后进行尿动力学研究，测量膀胱和尿道功能的变化。采用免疫组化(IHC)和Western blot检测CAV-1、cav -2、cav -3在大鼠尿道内的细胞定位和表达。结果:尿动力学实验显示膀胱炎组与对照组相比收缩间隔缩短(3.9±1.0 min vs 6.6±1.2 min)。结论:DO大鼠尿道CAV-1、-2和-3减少，提示cav可能与膀胱DO相关的尿道功能改变有关。

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来源期刊

International Neurourology Journal UROLOGY & NEPHROLOGY-

CiteScore

4.40

自引率

21.70%

发文量

审稿时长

4 weeks

期刊介绍： The International Neurourology Journal (Int Neurourol J, INJ) is a quarterly international journal that publishes high-quality research papers that provide the most significant and promising achievements in the fields of clinical neurourology and fundamental science. Specifically, fundamental science includes the most influential research papers from all fields of science and technology, revolutionizing what physicians and researchers practicing the art of neurourology worldwide know. Thus, we welcome valuable basic research articles to introduce cutting-edge translational research of fundamental sciences to clinical neurourology. In the editorials, urologists will present their perspectives on these articles. The original mission statement of the INJ was published on October 12, 1997. INJ provides authors a fast review of their work and makes a decision in an average of three to four weeks of receiving submissions. If accepted, articles are posted online in fully citable form. Supplementary issues will be published interim to quarterlies, as necessary, to fully allow berth to accept and publish relevant articles.