20-hydroxyecdysone reprograms amino acid metabolism to support the metamorphic development of Helicoverpa armigera.

IF 7.7 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2023-06-27 Epub Date: 2023-06-12 DOI:10.1016/j.celrep.2023.112644

Xiao-Pei Wang, Shu-Peng Sun, Yan-Xue Li, Lin Wang, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao

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引用次数: 1

Abstract

Amino acid metabolism is regulated according to nutrient conditions; however, the mechanism is not fully understood. Using the holometabolous insect cotton bollworm (Helicoverpa armigera) as a model, we report that hemolymph metabolites are greatly changed from the feeding larvae to the wandering larvae and to pupae. Arginine, alpha-ketoglutarate (α-KG), and glutamate (Glu) are identified as marker metabolites of feeding larvae, wandering larvae, and pupae, respectively. Arginine level is decreased by 20-hydroxyecdysone (20E) regulation via repression of argininosuccinate synthetase (Ass) expression and upregulation of arginase (Arg) expression during metamorphosis. α-KG is transformed from Glu by glutamate dehydrogenase (GDH) in larval midgut, which is repressed by 20E. The α-KG is then transformed to Glu by GDH-like in pupal fat body, which is upregulated by 20E. Thus, 20E reprogrammed amino acid metabolism during metamorphosis by regulating gene expression in a stage- and tissue-specific manner to support insect metamorphic development.

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20-羟基蜕皮激素重新编程氨基酸代谢以支持棉铃虫的变质发育。

氨基酸代谢是根据营养条件调节的；然而，人们还没有完全理解其机制。以全代谢组昆虫棉铃虫（Helicoverpa armigera）为模型，我们报道了血淋巴代谢产物从觅食幼虫到流浪幼虫再到蛹的巨大变化。精氨酸、α-酮戊二酸（α-KG）和谷氨酸（Glu）分别被鉴定为取食幼虫、游动幼虫和蛹的标记代谢产物。在变态过程中，20-羟基蜕皮激素（20E）通过抑制精氨酸琥珀酸合成酶（Ass）的表达和上调精氨酸酶（Arg）的分泌来降低精氨酸水平。α-KG是由谷氨酸脱氢酶（GDH）在幼虫中肠中转化而来的，被20E抑制。在蛹脂肪体中，α-KG通过GDH样转化为Glu，20E可上调其表达。因此，20E通过以阶段和组织特异性的方式调节基因表达，在变态过程中重新编程氨基酸代谢，以支持昆虫的变态发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.