Dynamic Changes in Circulatory Cytokines and Chemokines Levels in Mild to Severe COVID-19 Patients.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian Journal of Clinical Biochemistry Pub Date : 2023-04-01 DOI:10.1007/s12291-022-01108-x

Vandana Tiwari, Jyotsna Agarwal, Anumesh Kumar Pathak, Shivani Singh

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引用次数: 2

Abstract

Immune dysregulation is a key feature of the coronavirus disease-2019 (COVID-19). However, disparities in responses across ethnic groups are underappreciated. This study aimed to determine the relationship between chemokines and cytokines and the severity of COVID-19. Multiplex magnetic bead-based Luminex-100 was used to assess chemokine and cytokine levels in COVID-19 patients at admission (day-1) and after 4 days. The mean age of the patients recruited was 54.3 years, with 19 (63.3%) males. COVID-19 patients had significantly lower lymphocyte, monocyte, hemoglobin and eosinophil levels than controls (p < 0.05). COVID-19 patients showed significantly higher neutrophil levels than controls (p < 0.05). The baseline levels of IL-2, IL-6, IL-8, IL-10, and IFN-α/γ significantly increased in COVID-19 patients (p < 0.05). Chemokine levels (IP-10, MCP-1, MIG, and CCL-5) were significantly in COVID-19 patients. IL-8, IP-10, and MIG levels were significantly higher in the patients with severe COVID-19 (p < 0.05). Individuals with mild COVID-19 showed significantly higher levels of INF-α, IL-2, IL-6, and IL-8, whereas IL-10 levels were significantly lower (p < 0.05). TNF-levels decreased significantly in individuals with severe COVID-19, whereas IL-6, IL-8, and MIG levels increased (p < 0.05). After 4 days, INFα-, IL-2, IL-6, IL-8, IP-10, and MIG levels were significantly higher in patients with mild disease, whereas IL-6, MIG, and TNF-αlevels were significantly higher in patients with severe disease (p < 0.05). Thus, we conclude that COVID-19 is characterized by INF-α/γ, IL-6, IL-10, IP-10, MCP-1, MIG, and CCL5 dysregulation. IL-8, MIG, and IP-10 levels distinguish between moderate and severe COVID-19. Changes in INF-α, IL-2, IL-6, IL-8, IP-10, and MIG levels can be used to monitor disease progression.

Supplementary information: The online version contains supplementary material available at 10.1007/s12291-022-01108-x.

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轻至重度COVID-19患者循环细胞因子和趋化因子水平的动态变化

免疫失调是2019冠状病毒病(COVID-19)的一个关键特征。然而，不同种族群体的反应差异并未得到充分重视。本研究旨在确定趋化因子和细胞因子与COVID-19严重程度之间的关系。采用多路磁珠为基础的Luminex-100评估入院时(第1天)和入院后4天的趋化因子和细胞因子水平。患者平均年龄54.3岁，男性19例(63.3%)。与对照组相比，COVID-19患者淋巴细胞、单核细胞、血红蛋白和嗜酸性粒细胞水平显著降低(p p γ显著升高);COVID-19患者α/γ、IL-6、IL-10、IP-10、MCP-1、MIG和CCL5异常。IL-8、MIG和IP-10水平可区分中度和重度COVID-19。INF-α、IL-2、IL-6、IL-8、IP-10和MIG水平的变化可用于监测疾病进展。补充信息:在线版本包含补充资料，提供地址为10.1007/s12291-022-01108-x。

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来源期刊

Indian Journal of Clinical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.50

自引率

4.80%

发文量

期刊介绍： The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.