Effect of experimental toothpaste containing hydroxyapatite nanoparticles and propolis, with and without fluoride, on the microcosm biofilm and enamel demineralization.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2023-03-01 DOI:10.1080/08927014.2023.2217689

Caroline Fernanda da Silva Ribeiro, Aline Dionizio, Tamara Teodoro Araújo, Chelsea Maria Vilas Boas Feitosa Rodrigues, Amanda Costa Mattos, Letícia Galli Otaviano, Larissa Tercília Grizzo, Ana Carolina Magalhães, Marília Afonso Rabelo Buzalaf

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Abstract

This study evaluated the antimicrobial and anticaries effects of toothpaste containing hydroxyapatite nanoparticles (nanoHAP - 5 or 10%), xylitol (2 or 3%) and propolis (1 or 2%), associated or not with 1500 ppm fluoride (F). An in vitro model was used with microcosm biofilm produced from a pool of human saliva and McBain saliva (1:50) in the first 8 h of culture on 162 bovine enamel specimens. At the end of the experimental period, analyses of metabolic activity, colony forming units (CFU) and transverse microradiography (TMR) were performed. This study showed a possible decrease in demineralization and increase in remineralization by the commercial toothpaste (1500 ppm F) and for the experimental toothpaste containing the highest concentration of all agents, combined with F. In addition, a reduction in antimicrobial activity possibly caused by propolis and xylitol, mainly in relation to cariogenic bacteria, was observed.

查看原文本刊更多论文

含羟基磷灰石纳米颗粒和蜂胶实验牙膏加氟和不加氟对微观生物膜和牙釉质脱矿的影响。

本研究评估了含有羟基磷灰石纳米颗粒(nanoHAP - 5%或10%)、木糖醇(2%或3%)和蜂胶(1%或2%)的牙膏的抗菌和抗蛀牙效果，这些牙膏与1500ppm氟化物(F)相关或不相关。在162个牛牙釉质标本的培养前8小时内，用人类唾液和McBain唾液(1:50)产生的微生物生物膜建立了体外模型。实验结束时，进行代谢活性、菌落形成单位(CFU)和横向微放射照相(TMR)分析。本研究表明，商用牙膏(1500 ppm F)和实验牙膏(含所有剂浓度最高)与F的组合可能会降低脱矿和增加再矿化。此外，观察到蜂胶和木糖醇可能导致抗菌活性降低，主要与龋齿细菌有关。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.