Exosomal proteins as a source of biomarkers in colon cancer-derived peritoneal carcinomatosis - A pilot study.

IF 2.1 4区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

PROTEOMICS – Clinical Applications Pub Date : 2023-03-01 DOI:10.1002/prca.202100085

Paul A Vallejos, Ryan N Fuller, Janviere Kabagwira, Mei Li Kwong, Amber Gonda, James R W McMullen, Natasha Le, Matthew J Selleck, Lance D Miller, Christopher C Perry, Maheswari Senthil, Nathan R Wall

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引用次数: 1

Abstract

Purpose: Peritoneal carcinomatosis (PC), metastasized from colorectal cancer (CRC), remains a highly lethal disease. Outcomes of PC is significantly influenced by the amount of intra-abdominal tumor burden and therefore diagnostic tests that facilitate earlier diagnosis could improve PC treatment and patient outcomes.

Experimental design: Using mass-spectrometry-based proteomics, we characterized the protein features of circulating exosomes in the context of CRC PC, CRC with liver metastasis, and primary CRC limited to the colon. We profiled exosomes isolated from patient plasma to identify exosome-associated protein cargoes released by these cancer types.

Results: Analysis of the resulting data identified metastasis-specific exosome protein signatures. Bioinformatic analyses confirmed enrichment of proteins annotated to vesicle-associated processes and intracellular compartments, as well as representation of cancer hallmark functions and processes.

Conclusion and clinical relevance: This research yielded distinct protein profiles for the CRC patient groups and suggests the utility of plasma exosome proteomic analysis for a better understanding of PC development and metastasis.

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外泌体蛋白作为结肠癌源性腹膜癌的生物标志物来源-一项初步研究。

目的:腹膜癌(PC)，转移自结直肠癌(CRC)，仍然是一个高致死率的疾病。腹内肿瘤负荷显著影响PC的预后，因此早期诊断的诊断测试可以改善PC的治疗和患者预后。实验设计:利用基于质谱的蛋白质组学，我们表征了CRC PC、肝转移CRC和仅限于结肠的原发性CRC中循环外泌体的蛋白质特征。我们分析了从患者血浆中分离的外泌体，以鉴定这些癌症类型释放的外泌体相关蛋白。结果:分析所得数据确定了转移特异性外泌体蛋白特征。生物信息学分析证实了囊泡相关过程和细胞内区室的蛋白质富集，以及癌症标志功能和过程的代表。结论和临床意义:本研究为结直肠癌患者组提供了不同的蛋白质谱，并提示血浆外泌体蛋白质组学分析有助于更好地了解结直肠癌的发展和转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PROTEOMICS – Clinical Applications 医学-生化研究方法

CiteScore

5.20

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： PROTEOMICS - Clinical Applications has developed into a key source of information in the field of applying proteomics to the study of human disease and translation to the clinic. With 12 issues per year, the journal will publish papers in all relevant areas including: -basic proteomic research designed to further understand the molecular mechanisms underlying dysfunction in human disease -the results of proteomic studies dedicated to the discovery and validation of diagnostic and prognostic disease biomarkers -the use of proteomics for the discovery of novel drug targets -the application of proteomics in the drug development pipeline -the use of proteomics as a component of clinical trials.