A Free Fatty Acid Synthetic Agonist Accelerates Wound Healing and Improves Scar Quality in Mice.

IF 1.9 4区 医学 Q2 NURSING
Biological research for nursing Pub Date : 2023-07-01 Epub Date: 2022-11-29 DOI:10.1177/10998004221142331
Thais P Prado, Carlos P Jara, Vanessa C Dias Bóbbo, Rodrigo S Carraro, Davi Sidarta-Oliveira, Guilherme R A de Mendonça, Licio A Velloso, Eliana P Araújo
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引用次数: 0

Abstract

Background: Impaired wound healing is a health problem around the world, and the search for a novel product to repair wounded skin is a major topic in the field. GW9508 is a synthetic molecule described as a selective agonist of free fatty acid receptors (FFARs) 1 and 4, and there is evidence of its anti-inflammatory effects on several organs of the body.

Purpose: Here, we aimed to evaluate the effects of topical GW9508 on wound healing in mice.

Research design: First, we used bioinformatic methods to determine the expression of FFAR1 and FFAR4 mRNA in the skin from a human cell atlas assembled with single-cell transcriptomes. Next, we employed 6-week-old C57BL6J mice with 2 wounds inflicted in the back. The mice were randomly divided into 2 groups, a control group, which received topical vehicle, and a treatment group, which received GW9508, for 12 days. The wound was monitored by photographic documentation every 2 days, and samples were collected at day 6 and 12 post injury for RT-PCR, western blot and histology analyses.

Results: FFAR1 and FFAR4 mRNA are expressed in skin cells in similar amounts to those in other tissues. Topical GW9508 accelerated wound healing and decreased gene expression of IL-10 and metalloproteinase 9 on days 6 and 12 post injury. It increased the quantity of Collagen I and improved the organization of collagen fibres. Conclusions: Our results show that GW9508 could be an attractive drug treatment for wounded skin. Future studies need to be performed to assess the impact of GW9508 in chronic wound models.

一种游离脂肪酸合成激动剂可加速小鼠伤口愈合并改善疤痕质量。
背景:伤口愈合受损是全世界的一个健康问题,寻找一种新型产品来修复受伤的皮肤是该领域的一个重要课题。目的:在此,我们旨在评估外用 GW9508 对小鼠伤口愈合的影响:首先,我们使用生物信息学方法,从单细胞转录组组装的人类细胞图谱中确定皮肤中 FFAR1 和 FFAR4 mRNA 的表达。接着,我们用 6 周大的 C57BL6J 小鼠在背部造成 2 处伤口。小鼠被随机分为两组,对照组和治疗组,前者接受外用药物治疗,后者接受 GW9508 治疗,为期 12 天。每两天对伤口进行一次拍照记录,并在受伤后第 6 天和第 12 天采集样本进行 RT-PCR、Western 印迹和组织学分析:结果:FFAR1 和 FFAR4 mRNA 在皮肤细胞中的表达量与其他组织相似。外用 GW9508 可加速伤口愈合,并降低损伤后第 6 天和第 12 天 IL-10 和金属蛋白酶 9 的基因表达。它增加了胶原蛋白 I 的数量,改善了胶原纤维的组织。结论我们的研究结果表明,GW9508 可能是治疗皮肤损伤的一种有吸引力的药物。未来的研究需要评估 GW9508 对慢性伤口模型的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.10
自引率
4.00%
发文量
58
审稿时长
>12 weeks
期刊介绍: Biological Research For Nursing (BRN) is a peer-reviewed quarterly journal that helps nurse researchers, educators, and practitioners integrate information from many basic disciplines; biology, physiology, chemistry, health policy, business, engineering, education, communication and the social sciences into nursing research, theory and clinical practice. This journal is a member of the Committee on Publication Ethics (COPE)
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