Performance of two multiplex flow cytometric assays for antibody detection in Egyptian patients.

IF 1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

African Journal of Laboratory Medicine Pub Date : 2023-01-01 DOI:10.4102/ajlm.v12i1.2099

Alshymaa A Ahmed, Alia A El Shahawy, Heba M Kadry, Nora M Said

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Abstract

Background: Autoantibodies are vital biomarkers for the diagnosis, assessment and prognostic determination of various autoimmune disorders.

Objective: This study aimed to evaluate the performance of the two AtheNA Multi-Lyte^® systems for the detection of various autoantibodies.

Methods: A total of 105 systemic lupus erythematosus patients, 35 patients with other autoimmune diseases (diseased controls), and 30 healthy volunteers (healthy controls) at Zagazig University Hospitals, Zagazig city, Al Sharqia governorate were tested for anti-double-stranded DNA (anti-dsDNA) antibodies using indirect immunofluorescence (IIF) and the AtheNA Multi-Lyte^® anti-nuclear antibodies-II system between May 2020 and April 2022. Seventy-five patients with clinically suspected autoimmune vasculitis (AIV) and 25 healthy volunteers were also tested for anti-myeloperoxidase and anti-proteinase 3 antibodies using IIF, the AtheNA Multi-Lyte^® AIV system, and enzyme-linked immunosorbent assay (ELISA).

Results: The AtheNA anti-dsDNA test (98.5%) was more specific than IIF (96.9%) for diagnosing systemic lupus erythematosus, but both tests had the same sensitivity (38.1%). Combining both methods increased sensitivity to 47.6%, while increasing the cut-off of the AtheNA anti-dsDNA test to 134 international units/mL increased specificity to 100%. The AtheNA Multi-Lyte AIV system exhibited substantial agreement with IIF regarding anti-myeloperoxidase testing (κ = 0.65) and almost perfect agreement with ELISA (κ = 0.85). The AtheNA Multi-Lyte^® AIV system exhibited perfect agreement with IIF (κ = 1) and substantial agreement with ELISA for anti-proteinase 3 testing (κ = 0.63).

Conclusion: AtheNA Multi-Lyte^® systems appear to be reliable for anti-dsDNA, anti-myeloperoxidase, and anti-proteinase 3 screening and may be an optimal choice for monitoring anti-dsDNA levels.

What this study adds: It is necessary to evaluate various autoantibodies detection assays to increase both sensitivity and specificity of autoimmune diseases diagnostic approaches. AtheNA Multi-Lyte^® systems appear to be reliable for anti-dsDNA, anti-myeloperoxidase, and anti-proteinase 3 screening and may be an optimal choice for monitoring anti-dsDNA levels.

Abstract Image

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两种多重流式细胞术检测埃及患者抗体的性能。

背景:自身抗体是诊断、评估和判断各种自身免疫性疾病预后的重要生物标志物。目的:本研究旨在评估两种AtheNA Multi-Lyte®系统检测各种自身抗体的性能。方法:2020年5月至2022年4月，在Al Sharqia省Zagazig市Zagazig大学医院，采用间接免疫荧光(IIF)和AtheNA多lyte®抗核抗体- ii系统检测了105例系统性红斑狼疮患者、35例其他自身免疫性疾病患者(患病对照组)和30名健康志愿者(健康对照组)的抗双链DNA (anti-dsDNA)抗体。75例临床疑似自身免疫性血管炎(AIV)患者和25名健康志愿者也使用IIF、AtheNA Multi-Lyte®AIV系统和酶联免疫吸附试验(ELISA)检测抗髓过氧化物酶和抗蛋白酶3抗体。结果:AtheNA抗dsdna检测诊断系统性红斑狼疮的特异性(98.5%)高于IIF(96.9%)，但两种检测的敏感性相同(38.1%)。结合这两种方法可将灵敏度提高到47.6%，而将雅典娜抗dsdna检测的截止值提高到134国际单位/mL，将特异性提高到100%。AtheNA Multi-Lyte AIV系统在抗髓过氧化物酶测试方面与IIF表现出基本一致(κ = 0.65)，与ELISA几乎完全一致(κ = 0.85)。AtheNA Multi-Lyte®AIV系统与IIF (κ = 1)完全一致，与ELISA抗蛋白酶3检测(κ = 0.63)基本一致。结论:AtheNA Multi-Lyte®系统在抗dsdna、抗髓过氧化物酶和抗蛋白酶3筛选方面是可靠的，可能是监测抗dsdna水平的最佳选择。本研究补充:有必要评估各种自身抗体检测方法，以提高自身免疫性疾病诊断方法的敏感性和特异性。AtheNA Multi-Lyte®系统似乎是可靠的抗dsdna，抗髓过氧化物酶和抗蛋白酶3筛选，可能是监测抗dsdna水平的最佳选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

African Journal of Laboratory Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

12 weeks

期刊介绍： The African Journal of Laboratory Medicine, the official journal of ASLM, focuses on the role of the laboratory and its professionals in the clinical and public healthcare sectors,and is specifically based on an African frame of reference. Emphasis is on all aspects that promote and contribute to the laboratory medicine practices of Africa. This includes, amongst others: laboratories, biomedical scientists and clinicians, medical community, public health officials and policy makers, laboratory systems and policies (translation of laboratory knowledge, practices and technologies in clinical care), interfaces of laboratory with medical science, laboratory-based epidemiology, laboratory investigations, evidence-based effectiveness in real world (actual) settings.