Advances in Manufacturing Cardiomyocytes from Human Pluripotent Stem Cells.

IF 7.6 2区 工程技术 Q1 CHEMISTRY, APPLIED
Martha E Floy, Fathima Shabnam, Aaron D Simmons, Vijesh J Bhute, Gyuhyung Jin, Will A Friedrich, Alexandra B Steinberg, Sean P Palecek
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引用次数: 2

Abstract

The emergence of human pluripotent stem cell (hPSC) technology over the past two decades has provided a source of normal and diseased human cells for a wide variety of in vitro and in vivo applications. Notably, hPSC-derived cardiomyocytes (hPSC-CMs) are widely used to model human heart development and disease and are in clinical trials for treating heart disease. The success of hPSC-CMs in these applications requires robust, scalable approaches to manufacture large numbers of safe and potent cells. Although significant advances have been made over the past decade in improving the purity and yield of hPSC-CMs and scaling the differentiation process from 2D to 3D, efforts to induce maturation phenotypes during manufacturing have been slow. Process monitoring and closed-loop manufacturing strategies are just being developed. We discuss recent advances in hPSC-CM manufacturing, including differentiation process development and scaling and downstream processes as well as separation and stabilization.

Abstract Image

Abstract Image

人多能干细胞制造心肌细胞的研究进展。
在过去的二十年中,人类多能干细胞(hPSC)技术的出现为各种体外和体内应用提供了正常和患病人类细胞的来源。值得注意的是,hpsc来源的心肌细胞(hPSC-CMs)被广泛用于模拟人类心脏发育和疾病,并在治疗心脏病的临床试验中。hPSC-CMs在这些应用中的成功需要强大的、可扩展的方法来制造大量安全和有效的细胞。尽管在过去十年中,在提高hPSC-CMs的纯度和产量以及将分化过程从2D扩展到3D方面取得了重大进展,但在制造过程中诱导成熟表型的努力进展缓慢。过程监控和闭环制造策略才刚刚发展起来。我们讨论了hPSC-CM制造的最新进展,包括差异化工艺开发和规模化以及下游工艺以及分离和稳定。
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来源期刊
Annual review of chemical and biomolecular engineering
Annual review of chemical and biomolecular engineering CHEMISTRY, APPLIED-ENGINEERING, CHEMICAL
CiteScore
16.00
自引率
0.00%
发文量
25
期刊介绍: The Annual Review of Chemical and Biomolecular Engineering aims to provide a perspective on the broad field of chemical (and related) engineering. The journal draws from disciplines as diverse as biology, physics, and engineering, with development of chemical products and processes as the unifying theme.
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