In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine cells.

IF 1.5 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Veterinary Science Pub Date : 2023-05-01 DOI:10.4142/jvs.23007

Dong-Ha Lee, Eun-Bee Lee, Jong-Pil Seo, Eun-Ju Ko

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Abstract

Background: Toll-like receptor (TLR) agonists have been used as adjuvants to modulate immune responses in both animals and humans.

Objectives: The objective of this study was to evaluate the combined effects of the TLR 4 agonist monophosphoryl lipid A (MPL) and the TLR 3 agonist polyinosinic:polycytidylic acid (Poly I:C) on equine peripheral blood mononuclear cells (PBMCs), monocyte-derived dendritic cells (MoDCs), and bone marrow-derived mesenchymal stromal cells (BM-MSCs).

Methods: The PBMCs, MoDCs, and BM-MSCs collected from three mixed breed horses were treated with MPL, Poly I:C, and their combination. The mRNA expression of interferon gamma (IFN-γ), interleukin (IL)-1β, IL-4, IL-6, IL-8, IL-12p40, tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) was determined using real-time polymerase chain reaction.

Results: The combination of MPL and Poly I:C significantly upregulated immunomodulatory responses in equine cells/ without cytotoxicity. The combination induced greater mRNA expression of pro-inflammatory cytokines IFN-γ and IL-6 than MPL or Poly I:C stimulation alone in PBMCs. In addition, the combination induced significantly higher mRNA expression of IL-1β, IL-6, and IL-12p40 in MoDCs, and IL-8, MCP-1, and VEGF in BM-MSCs compared to stimulation with a single TLR agonist.

Conclusions: The combination of MPL and Poly I:C can be used as a potential adjuvant candidate for vaccines to aid in preventing infectious diseases in horses.

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单磷酰脂A和聚I:C联合对马细胞的体外影响。

背景:toll样受体(TLR)激动剂已被用作佐剂来调节动物和人类的免疫反应。目的:本研究的目的是评估tlr4激动剂单磷酰脂质A (MPL)和tlr3激动剂多肌苷:多胞酸(Poly I:C)对马外周血单核细胞(PBMCs)、单核细胞源性树突状细胞(MoDCs)和骨髓源性间充质基质细胞(BM-MSCs)的联合作用。方法:采用MPL、Poly I:C及其组合对3匹杂交马的PBMCs、MoDCs和BM-MSCs进行处理。实时聚合酶链式反应检测干扰素γ (IFN-γ)、白细胞介素(IL)-1β、IL-4、IL-6、IL-8、IL-12p40、肿瘤坏死因子α (TNF-α)、血管内皮生长因子(VEGF)、单核细胞趋化蛋白-1 (MCP-1) mRNA表达。结果:MPL和Poly I:C联合使用可显著上调马细胞的免疫调节反应/无细胞毒性。与MPL或Poly I:C单独刺激相比，MPL联合刺激在PBMCs中诱导了更大的促炎细胞因子IFN-γ和IL-6的mRNA表达。此外，与单一TLR激动剂刺激相比，联合使用可显著提高MoDCs中IL-1β、IL-6和IL-12p40的mRNA表达，以及BM-MSCs中IL-8、MCP-1和VEGF的mRNA表达。结论:MPL与Poly I:C联合可作为马传染病预防疫苗的潜在佐剂候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Science 农林科学-兽医学

CiteScore

3.10

自引率

5.60%

发文量

审稿时长

1.3 months

期刊介绍： The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.