Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., Natrinema zhouii sp. nov., extremely halophilic archaea isolated from marine environments and a salt mine.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2023-03-31 DOI:10.1007/s00792-023-01294-3

Yao Hu, Xue Ma, Xin-Xin Li, Shun Tan, Mu Cheng, Jing Hou, Heng-Lin Cui

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引用次数: 2

Abstract

Four extremely halophilic archaeal strains (ZJ2^T, BND6^T, DT87^T, and YPL30^T) were isolated from marine environments and a salt mine in China. The 16S rRNA and rpoB' gene sequence similarities among strains ZJ2^T, BND6^T, DT87^T, YPL30^T and the current species of Natrinema were 93.2-99.3% and 89.2-95.8%, respectively. Both phylogenetic and phylogenomic analyses revealed that strains ZJ2^T, BND6^T, DT87^T, and YPL30^T cluster with the Natrinema members. The overall genome-related indexes (ANI, isDDH, and AAI) among these four strains and the current species of genus Natrinema were 70-88%, 22-43% and 75-89%, respectively, clearly below the threshold values for species boundary. Strains ZJ2^T, BND6^T, DT87^T, and YPL30^T could be distinguished from the related species according to differential phenotypic characteristics. The major polar lipids of the four strains were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and disulfated mannosyl glucosyl diether (S₂-DGD). The phenotypic, chemotaxonomic, phylogenetic and phylogenomic features indicated that strains ZJ2^T (= CGMCC 1.18786^T = JCM 34918^T), BND6^T (= CGMCC 1.18777^T = JCM 34909^T), DT87^T (= CGMCC 1.18921^T = JCM 35420^T), and YPL30^T (= CGMCC 1.15337^T = JCM 31113^T) represent four novel species of the genus Natrinema, for which the names, Natrinema caseinilyticum sp. nov., Natrinema gelatinilyticum sp. nov., Natrinema marinum sp. nov., and Natrinema zhouii sp. nov., are proposed.

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溶干酪藻Natrinema caseinicum sp.nov.，溶胶藻Natrinima glugelicum sp.nov.，海洋藻Natrineman marinum sp.nov..，从海洋环境和盐矿中分离的极嗜盐古菌。

从中国海洋环境和盐矿中分离到4株极端嗜盐古细菌(ZJ2T、BND6T、DT87T和YPL30T)。菌株ZJ2T、BND6T、DT87T、YPL30T与现种Natrinema的16S rRNA和rpoB’基因序列相似性分别为93.2 ~ 99.3%和89.2 ~ 95.8%。系统发育和系统基因组分析表明，菌株ZJ2T、BND6T、DT87T和YPL30T与Natrinema成员聚类。4个菌株与Natrinema属现有种的总体基因组相关指数ANI、isDDH和AAI分别为70 ~ 88%、22 ~ 43%和75 ~ 89%，均低于种界阈值。菌株ZJ2T、BND6T、DT87T和YPL30T根据差异表型特征可与亲缘种区分。4株菌株的主要极性脂质为磷脂酸(PA)、磷脂酰甘油(PG)、磷脂酰甘油磷酸甲酯(PGP-Me)、硫酸甘露糖基葡萄糖二醚(S-DGD-1)和硫酸甘露糖基葡萄糖二醚(S2-DGD)。chemotaxonomic表型,系统发育和phylogenomic特性表明,菌株ZJ2T (= CGMCC 1.18786 T = JCM 34918 T), BND6T (= CGMCC 1.18777 T = JCM 34909 T), DT87T (= CGMCC 1.18921 T = JCM 35420 T),和YPL30T JCM 31113 (= CGMCC 1.15337 T = T)代表四个属的新物种Natrinema,的名字,Natrinema caseinilyticum sp. 11月,Natrinema gelatinilyticum sp. 11月,Natrinema marinum sp. 11月,和Natrinema zhouii sp. 11月,建议。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464