Characterization and In-vitro Study of Micro-encapsulation Chitosan Alginate of Single-bulb Garlic Extract.

Q2 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical nanotechnology Pub Date : 2024-01-01 DOI:10.2174/2211738511666230607121118

Sri Rahayu Lestari, Abdul Gofur, Dra Hartatiek, Yuslinda Annisa, Dimas Nur Ramadhani, Amalia Nur Rahma, Dahniar Nur Aisyah, Ikfi Nihayatul Mufidah, Nadiya Dini Rifqi

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引用次数: 0

Abstract

Background: Single-bulb garlic extract (SBGE) contains more active compounds than regular garlic, but it is unstable and easily degraded in the digestive tract. SBGE is expected to be protected by microencapsulation chitosan-alginate (MCA).

Objective: The present study aimed to characterize and assess the antioxidant activity, hemocompatibility, and toxicity of MCA-SBGE in 3T3-L1 cells.

Methods: The research procedures consist of extraction of single bulb garlic, preparation of MCASBGE, Particle Size Analyzer (PSA), FTIR analysis, DPPH assay, hemocompatibility test, and MTT assay.

Results: The average size of MCA-SGBE was 423.7 ± 2.8 nm, the polydispersity index (PdI) was 0.446 ± 0.022, and the zeta potential was -24.5 ± 0.4 mV. MCA-SGBE was spherical with a diameter range of 0.65-0.9 μm. A shift in absorption and addition of functional groups was found in SBGE after encapsulation. MCA-SBGE, at a concentration of 24 x 10³ ppm, has higher antioxidants than SBGE. The hemocompatibility test shows the hemolysis of MCA-SBGE lower than SBGE. MCA-SBGE was not toxic to 3T3-L1 cells with cell viability percentage above 100% at all concentrations.

Conclusion: MCA-SBGE characterization has microparticle criteria with homogeneous PdI values, low particle stability, and spherical morphology. The results showed that SBGE and MCA-SBGE are nonhemolytic, compatible with red blood cells, and non-toxic to 3T3-L1 cells.

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单粒大蒜提取物壳聚糖藻酸盐微胶囊的表征和体外研究

背景：单球大蒜提取物（SBGE）比普通大蒜含有更多的活性化合物，但它不稳定，容易在消化道中降解。单球大蒜提取物有望受到壳聚糖-精氨酸微胶囊（MCA）的保护：本研究旨在表征和评估 MCA-SBGE 在 3T3-L1 细胞中的抗氧化活性、血液相容性和毒性：研究程序包括提取单球大蒜、制备 MCA-SBGE、粒度分析仪（PSA）、傅立叶变换红外光谱分析、DPPH 试验、血液相容性试验和 MTT 试验：MCA-SGBE 的平均粒径为 423.7 ± 2.8 nm，多分散指数（PdI）为 0.446 ± 0.022，Zeta 电位为 -24.5 ± 0.4 mV。MCA-SGBE 呈球形，直径范围为 0.65-0.9 μm。封装后，SBGE 的吸收发生了变化，并增加了官能团。MCA-SBGE 的抗氧化剂浓度为 24 x 103 ppm，高于 SBGE。血液相容性测试表明，MCA-SBGE 的溶血率低于 SBGE。MCA-SBGE 对 3T3-L1 细胞无毒性，在所有浓度下细胞存活率都高于 100%：结论：MCA-SBGE 具有微颗粒标准，PdI 值均匀，颗粒稳定性低，形态呈球形。结果表明，SBGE 和 MCA-SBGE 不溶血，与红细胞相容，对 3T3-L1 细胞无毒。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.