Positron Emission Tomography-Computed Tomography Imaging of Selective Lobar Delivery of Stem Cells in Ex Vivo Lung Model of Mechanical Ventilation.

IF 2 4区 医学 Q3 RESPIRATORY SYSTEM
Luiz Maracaja, Ashish K Khanna, Sean V Murphy, Danielle L V Maracaja, Magan R Lane, Oula Khoury, Josh Tan, Naresh Damuka, Freda F Crawford, Joseph A Bottoms, Mack D Miller, David W Kaczka, James Eric Jordam, Kiran Kumar Solingapuram Sai
{"title":"Positron Emission Tomography-Computed Tomography Imaging of Selective Lobar Delivery of Stem Cells in <i>Ex Vivo</i> Lung Model of Mechanical Ventilation.","authors":"Luiz Maracaja,&nbsp;Ashish K Khanna,&nbsp;Sean V Murphy,&nbsp;Danielle L V Maracaja,&nbsp;Magan R Lane,&nbsp;Oula Khoury,&nbsp;Josh Tan,&nbsp;Naresh Damuka,&nbsp;Freda F Crawford,&nbsp;Joseph A Bottoms,&nbsp;Mack D Miller,&nbsp;David W Kaczka,&nbsp;James Eric Jordam,&nbsp;Kiran Kumar Solingapuram Sai","doi":"10.1089/jamp.2022.0013","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Introduction:</i></b> The delivery of cell therapies may be an important frontier to treat different respiratory diseases in the near future. However, the cell size, delivery conditions, cell viability, and effect in the pulmonary function are critical factors. We performed a proof-of-concept experiment using <i>ex vivo</i> lungs and novel subglottic airway device that allows for selective lobar isolation and administration of drugs and biologics in liquid solution deep into the lung tissues, while simultaneously ventilating the rest of the lung lobes. <b><i>Methods:</i></b> We used radiolabeled cells and positron emission tomography-computed tomography (PET-CT) imaging to demonstrate the feasibility of high-yield cell delivery to a specifically targeted lobe. This study proposes an alternative delivery method of live cells labeled with radioactive isotope into the lung parenchyma and tracks the cell delivery using PET-CT imaging. The technique combines selective lobar isolation and lobar infusion to carry large particles distal to the trachea, subtending bronchial segments and reaching alveoli in targeted regions. <b><i>Results:</i></b> The solution with cells and carrier achieved a complete and homogeneous lobar distribution. An increase in tissue density was shown on the computed tomography (CT) scan, and the PET-CT imaging demonstrated retention of the activity at central, peripheral lung parenchyma, and pleural surface. The increase in CT density and metabolic activity of the isotope was restricted to the desired lobe only without leak to other lobes. <b><i>Conclusion:</i></b> The selective lobe delivery is targeted and imaging-guided by bronchoscopy and CT to a specific diseased lobe during mechanical ventilation. The feasibility of high-yield cell delivery demonstrated in this study will lead to the development of potential novel therapies that contribute to lung health.</p>","PeriodicalId":14940,"journal":{"name":"Journal of Aerosol Medicine and Pulmonary Drug Delivery","volume":"36 1","pages":"20-26"},"PeriodicalIF":2.0000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942179/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Aerosol Medicine and Pulmonary Drug Delivery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1089/jamp.2022.0013","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: The delivery of cell therapies may be an important frontier to treat different respiratory diseases in the near future. However, the cell size, delivery conditions, cell viability, and effect in the pulmonary function are critical factors. We performed a proof-of-concept experiment using ex vivo lungs and novel subglottic airway device that allows for selective lobar isolation and administration of drugs and biologics in liquid solution deep into the lung tissues, while simultaneously ventilating the rest of the lung lobes. Methods: We used radiolabeled cells and positron emission tomography-computed tomography (PET-CT) imaging to demonstrate the feasibility of high-yield cell delivery to a specifically targeted lobe. This study proposes an alternative delivery method of live cells labeled with radioactive isotope into the lung parenchyma and tracks the cell delivery using PET-CT imaging. The technique combines selective lobar isolation and lobar infusion to carry large particles distal to the trachea, subtending bronchial segments and reaching alveoli in targeted regions. Results: The solution with cells and carrier achieved a complete and homogeneous lobar distribution. An increase in tissue density was shown on the computed tomography (CT) scan, and the PET-CT imaging demonstrated retention of the activity at central, peripheral lung parenchyma, and pleural surface. The increase in CT density and metabolic activity of the isotope was restricted to the desired lobe only without leak to other lobes. Conclusion: The selective lobe delivery is targeted and imaging-guided by bronchoscopy and CT to a specific diseased lobe during mechanical ventilation. The feasibility of high-yield cell delivery demonstrated in this study will lead to the development of potential novel therapies that contribute to lung health.

Abstract Image

Abstract Image

Abstract Image

机械通气离体肺模型中干细胞选择性大叶递送的正电子发射断层扫描成像。
在不久的将来,细胞递送疗法可能是治疗不同呼吸系统疾病的一个重要前沿。然而,细胞大小、输送条件、细胞活力和对肺功能的影响是关键因素。我们使用离体肺和新型声门下气道设备进行了概念验证实验,该设备允许选择性肺叶隔离,并将液体溶液中的药物和生物制剂注入肺组织深处,同时对其余肺叶进行通气。方法:我们使用放射性标记细胞和正电子发射断层扫描-计算机断层扫描(PET-CT)成像来证明高产量细胞递送到特定目标叶的可行性。本研究提出了一种放射性同位素标记活细胞进入肺实质的替代递送方法,并使用PET-CT成像跟踪细胞递送。该技术结合选择性肺叶分离和肺叶输注,将大颗粒运送到气管远端,沿支气管段到达目标区域的肺泡。结果:细胞和载体的溶液实现了完整均匀的脑叶分布。计算机断层扫描(CT)显示组织密度增加,PET-CT成像显示中央,周围肺实质和胸膜表面的活动保留。CT密度和同位素代谢活性的增加仅限于所需叶,而不会泄漏到其他叶。结论:在机械通气过程中,支气管镜和CT的成像引导下,选择性肺叶输送是有针对性的。本研究证明的高产量细胞递送的可行性将导致有助于肺部健康的潜在新疗法的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.70
自引率
2.90%
发文量
34
审稿时长
>12 weeks
期刊介绍: Journal of Aerosol Medicine and Pulmonary Drug Delivery is the only peer-reviewed journal delivering innovative, authoritative coverage of the health effects of inhaled aerosols and delivery of drugs through the pulmonary system. The Journal is a forum for leading experts, addressing novel topics such as aerosolized chemotherapy, aerosolized vaccines, methods to determine toxicities, and delivery of aerosolized drugs in the intubated patient. Journal of Aerosol Medicine and Pulmonary Drug Delivery coverage includes: Pulmonary drug delivery Airway reactivity and asthma treatment Inhalation of particles and gases in the respiratory tract Toxic effects of inhaled agents Aerosols as tools for studying basic physiologic phenomena.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信